TY - JOUR
T1 - Toward Light-Regulated Living Biomaterials
AU - Sankaran, Shrikrishnan
AU - Zhao, Shifang
AU - Muth, Christina
AU - Paez, Julieta
AU - del Campo, Aránzazu
N1 - Funding Information:
S.S. and S.Z. contributed equally to this work. The authors thank Rebecca Ludwig for her aid in the synthesis of PA-IPTG, Dr. Marcus Koch for his help with the SEM imaging and Prof. Andres Garcia for providing the MEF-vincGFP cells and constructive suggestions. A.d.C. and S.S. are grateful for funding from the SFB1027 collective research center for this project. China Scholarship Council (CSC) is acknowledged for its financial support to S.Z. A.d.C. and J.P. acknowledge funding from the European Union’s Horizon 2020 research and innovation programme under the FET PROACTIVE Grant Agreement No. 731957 (MECHANO-CONTROL).
Funding Information:
S.S. and S.Z. contributed equally to this work. The authors thank Rebecca Ludwig for her aid in the synthesis of PA-IPTG, Dr. Marcus Koch for his help with the SEM imaging and Prof. Andres Garcia for providing the MEF-vincGFP cells and constructive suggestions. A.d.C. and S.S. are grateful for funding from the SFB1027 collective research center for this project. China Scholarship Council (CSC) is acknowledged for its financial support to S.Z. A.d.C. and J.P. acknowledge funding from the European Union's Horizon 2020 research and innovation programme under the FET PROACTIVE Grant Agreement No. 731957 (MECHANO-CONTROL).
Publisher Copyright:
© 2018 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/8
Y1 - 2018/8
N2 - Living materials are an emergent material class, infused with the productive, adaptive, and regenerative properties of living organisms. Property regulation in living materials requires encoding responsive units in the living components to allow external manipulation of their function. Here, an optoregulated Escherichia coli (E. coli)-based living biomaterial that can be externally addressed using light to interact with mammalian cells is demonstrated. This is achieved by using a photoactivatable inducer of gene expression and bacterial surface display technology to present an integrin-specific miniprotein on the outer membrane of an endotoxin-free E. coli strain. Hydrogel surfaces functionalized with the bacteria can expose cell adhesive molecules upon in situ light-activation, and trigger cell adhesion. Surface immobilized bacteria are able to deliver a fluorescent protein to the mammalian cells with which they are interacting, indicating the potential of such a bacterial material to deliver molecules to cells in a targeted manner.
AB - Living materials are an emergent material class, infused with the productive, adaptive, and regenerative properties of living organisms. Property regulation in living materials requires encoding responsive units in the living components to allow external manipulation of their function. Here, an optoregulated Escherichia coli (E. coli)-based living biomaterial that can be externally addressed using light to interact with mammalian cells is demonstrated. This is achieved by using a photoactivatable inducer of gene expression and bacterial surface display technology to present an integrin-specific miniprotein on the outer membrane of an endotoxin-free E. coli strain. Hydrogel surfaces functionalized with the bacteria can expose cell adhesive molecules upon in situ light-activation, and trigger cell adhesion. Surface immobilized bacteria are able to deliver a fluorescent protein to the mammalian cells with which they are interacting, indicating the potential of such a bacterial material to deliver molecules to cells in a targeted manner.
KW - dynamic biomaterials
KW - endotoxin-free E. coli
KW - living biointerfaces
KW - optogenetics
UR - https://www.scopus.com/pages/publications/85051477808
U2 - 10.1002/advs.201800383
DO - 10.1002/advs.201800383
M3 - Article
AN - SCOPUS:85051477808
SN - 2198-3844
VL - 5
JO - Advanced science
JF - Advanced science
IS - 8
M1 - 1800383
ER -