Toward microfluidic sperm refinement: continuous flow label-free analysis and sorting of sperm cells

B. de Wagenaar, Stefan Dekker, Albert van den Berg, Loes Irene Segerink

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    Abstract

    This manuscript reports upon the development of a microfluidic setup to detect and sort sperm cells from polystyrene beads label-free and non-invasively. Detection is performed by impedance analysis. When sperm cells passed the microelectrodes, the recorded impedance (19.6 ± 5.7 Ω) was higher compared to the impedance change of passing 3 μm beads (5.4 ± 0.7 Ω ). This information was used to discriminate and actively sort beads and spermatozoa using dielectrophoresis at a rate of <1 cell/bead s-1.
    Original languageUndefined
    Title of host publicationThe 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015)
    PublisherChemical and Biological Micro Systems Society
    Pages528-530
    Number of pages3
    ISBN (Print)978-0-9798064-8-3
    Publication statusPublished - 25 Oct 2015
    Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of
    Duration: 25 Oct 201529 Oct 2015
    Conference number: 19
    http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

    Publication series

    NameProceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS
    PublisherChemical and Biological Microsystems Society
    ISSN (Print)1556-5904

    Conference

    Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
    Abbreviated titleMicroTas 2015
    CountryKorea, Republic of
    CityGyeongju
    Period25/10/1529/10/15
    Internet address

    Keywords

    • EWI-26551
    • METIS-315086
    • IR-98701

    Cite this

    de Wagenaar, B., Dekker, S., van den Berg, A., & Segerink, L. I. (2015). Toward microfluidic sperm refinement: continuous flow label-free analysis and sorting of sperm cells. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015) (pp. 528-530). (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS). Chemical and Biological Micro Systems Society.