Toward microfluidic sperm refinement: continuous flow label-free analysis and sorting of sperm cells

B. de Wagenaar; Stefan Dekker; Albert van den Berg; Loes Irene Segerink

Toward microfluidic sperm refinement: continuous flow label-free analysis and sorting of sperm cells

B. de Wagenaar, Stefan Dekker, Albert van den Berg, Loes Irene Segerink

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

This manuscript reports upon the development of a microfluidic setup to detect and sort sperm cells from polystyrene beads label-free and non-invasively. Detection is performed by impedance analysis. When sperm cells passed the microelectrodes, the recorded impedance (19.6 ± 5.7 Ω) was higher compared to the impedance change of passing 3 μm beads (5.4 ± 0.7 Ω ). This information was used to discriminate and actively sort beads and spermatozoa using dielectrophoresis at a rate of <1 cell/bead s-1.

Original language	Undefined
Title of host publication	The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015)
Publisher	Chemical and Biological Micro Systems Society
Pages	528-530
Number of pages	3
ISBN (Print)	978-0-9798064-8-3
Publication status	Published - 25 Oct 2015
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 Conference number: 19 http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Publication series

Name	Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS
Publisher	Chemical and Biological Microsystems Society
ISSN (Print)	1556-5904

Conference

Conference	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
Abbreviated title	MicroTas 2015
Country	Korea, Republic of
City	Gyeongju
Period	25/10/15 → 29/10/15
Internet address	http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Keywords

EWI-26551
METIS-315086
IR-98701

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Cite this

de Wagenaar, B., Dekker, S., van den Berg, A., & Segerink, L. I. (2015). Toward microfluidic sperm refinement: continuous flow label-free analysis and sorting of sperm cells. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015) (pp. 528-530). (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS). Chemical and Biological Micro Systems Society.

de Wagenaar, B. ; Dekker, Stefan ; van den Berg, Albert ; Segerink, Loes Irene. / Toward microfluidic sperm refinement: continuous flow label-free analysis and sorting of sperm cells. The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). Chemical and Biological Micro Systems Society, 2015. pp. 528-530 (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).

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abstract = "This manuscript reports upon the development of a microfluidic setup to detect and sort sperm cells from polystyrene beads label-free and non-invasively. Detection is performed by impedance analysis. When sperm cells passed the microelectrodes, the recorded impedance (19.6 ± 5.7 Ω) was higher compared to the impedance change of passing 3 μm beads (5.4 ± 0.7 Ω ). This information was used to discriminate and actively sort beads and spermatozoa using dielectrophoresis at a rate of <1 cell/bead s-1.",

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de Wagenaar, B, Dekker, S, van den Berg, A & Segerink, LI 2015, Toward microfluidic sperm refinement: continuous flow label-free analysis and sorting of sperm cells. in The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS, Chemical and Biological Micro Systems Society, pp. 528-530, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, Gyeongju, Korea, Republic of, 25/10/15.

Toward microfluidic sperm refinement: continuous flow label-free analysis and sorting of sperm cells. / de Wagenaar, B.; Dekker, Stefan; van den Berg, Albert ; Segerink, Loes Irene.

The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). Chemical and Biological Micro Systems Society, 2015. p. 528-530 (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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N2 - This manuscript reports upon the development of a microfluidic setup to detect and sort sperm cells from polystyrene beads label-free and non-invasively. Detection is performed by impedance analysis. When sperm cells passed the microelectrodes, the recorded impedance (19.6 ± 5.7 Ω) was higher compared to the impedance change of passing 3 μm beads (5.4 ± 0.7 Ω ). This information was used to discriminate and actively sort beads and spermatozoa using dielectrophoresis at a rate of <1 cell/bead s-1.

AB - This manuscript reports upon the development of a microfluidic setup to detect and sort sperm cells from polystyrene beads label-free and non-invasively. Detection is performed by impedance analysis. When sperm cells passed the microelectrodes, the recorded impedance (19.6 ± 5.7 Ω) was higher compared to the impedance change of passing 3 μm beads (5.4 ± 0.7 Ω ). This information was used to discriminate and actively sort beads and spermatozoa using dielectrophoresis at a rate of <1 cell/bead s-1.

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de Wagenaar B, Dekker S, van den Berg A , Segerink LI. Toward microfluidic sperm refinement: continuous flow label-free analysis and sorting of sperm cells. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS 2015). Chemical and Biological Micro Systems Society. 2015. p. 528-530. (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).