Towards 4D Printed Scaffolds for Tissue Engineering: Exploiting 3D Shape Memory Polymers to Deliver Time-Controlled Stimulus on Cultured Cells

Wilhelmus J. Hendrikson, Jeroen Rouwkema, Federico Clementi, Clemens van Blitterswijk, Silvia Farè, Lorenzo Moroni

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized by dynamic-mechanical analysis. The thermo-mechanical analysis showed a T g at about 32 °C (T g = T trans), indicating no influence of the fabrication process on the transition temperature. In addition, shape recovery tests showed a good recovery of the permanent shape for both scaffold configurations. When cells were seeded onto the scaffolds in the temporary shape and the permanent shape was recovered, cells were significantly more elongated after shape recovery. Thus, the mechanical stimulus imparted by shape recovery is able to influence the shape of cells and nuclei. The obtained results indicate that a single mechanical stimulus is sufficient to initiate changes in the morphology of adherent cells.
Original languageEnglish
Article number031001
JournalBiofabrication
Volume9
Issue number3
DOIs
Publication statusPublished - 2 Aug 2017

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Tissue Engineering
Scaffolds (biology)
Shape memory effect
Tissue engineering
Scaffolds
Cultured Cells
Polymers
Cells
Recovery
Cell Nucleus Shape
3D printers
Polyurethanes
Transition Temperature
Dynamic mechanical analysis
Superconducting transition temperature
Cues
Technology
Fabrication

Cite this

Hendrikson, Wilhelmus J. ; Rouwkema, Jeroen ; Clementi, Federico ; van Blitterswijk, Clemens ; Farè, Silvia ; Moroni, Lorenzo. / Towards 4D Printed Scaffolds for Tissue Engineering : Exploiting 3D Shape Memory Polymers to Deliver Time-Controlled Stimulus on Cultured Cells. In: Biofabrication. 2017 ; Vol. 9, No. 3.
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abstract = "Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized by dynamic-mechanical analysis. The thermo-mechanical analysis showed a T g at about 32 °C (T g = T trans), indicating no influence of the fabrication process on the transition temperature. In addition, shape recovery tests showed a good recovery of the permanent shape for both scaffold configurations. When cells were seeded onto the scaffolds in the temporary shape and the permanent shape was recovered, cells were significantly more elongated after shape recovery. Thus, the mechanical stimulus imparted by shape recovery is able to influence the shape of cells and nuclei. The obtained results indicate that a single mechanical stimulus is sufficient to initiate changes in the morphology of adherent cells.",
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Towards 4D Printed Scaffolds for Tissue Engineering : Exploiting 3D Shape Memory Polymers to Deliver Time-Controlled Stimulus on Cultured Cells. / Hendrikson, Wilhelmus J.; Rouwkema, Jeroen ; Clementi, Federico ; van Blitterswijk, Clemens; Farè, Silvia; Moroni, Lorenzo.

In: Biofabrication, Vol. 9, No. 3, 031001, 02.08.2017.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Hendrikson, Wilhelmus J.

AU - Rouwkema, Jeroen

AU - Clementi, Federico

AU - van Blitterswijk, Clemens

AU - Farè, Silvia

AU - Moroni, Lorenzo

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AB - Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized by dynamic-mechanical analysis. The thermo-mechanical analysis showed a T g at about 32 °C (T g = T trans), indicating no influence of the fabrication process on the transition temperature. In addition, shape recovery tests showed a good recovery of the permanent shape for both scaffold configurations. When cells were seeded onto the scaffolds in the temporary shape and the permanent shape was recovered, cells were significantly more elongated after shape recovery. Thus, the mechanical stimulus imparted by shape recovery is able to influence the shape of cells and nuclei. The obtained results indicate that a single mechanical stimulus is sufficient to initiate changes in the morphology of adherent cells.

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