Towards 4D Printed Scaffolds for Tissue Engineering: Exploiting 3D Shape Memory Polymers to Deliver Time-Controlled Stimulus on Cultured Cells

Wilhelmus J. Hendrikson, Jeroen Rouwkema, Federico Clementi, Clemens van Blitterswijk, Silvia Farè, Lorenzo Moroni

    Research output: Contribution to journalArticleAcademicpeer-review

    26 Citations (Scopus)

    Abstract

    Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized by dynamic-mechanical analysis. The thermo-mechanical analysis showed a T g at about 32 °C (T g = T trans), indicating no influence of the fabrication process on the transition temperature. In addition, shape recovery tests showed a good recovery of the permanent shape for both scaffold configurations. When cells were seeded onto the scaffolds in the temporary shape and the permanent shape was recovered, cells were significantly more elongated after shape recovery. Thus, the mechanical stimulus imparted by shape recovery is able to influence the shape of cells and nuclei. The obtained results indicate that a single mechanical stimulus is sufficient to initiate changes in the morphology of adherent cells.
    Original languageEnglish
    Article number031001
    JournalBiofabrication
    Volume9
    Issue number3
    DOIs
    Publication statusPublished - 2 Aug 2017

    Fingerprint

    Tissue Engineering
    Scaffolds (biology)
    Shape memory effect
    Tissue engineering
    Scaffolds
    Cultured Cells
    Polymers
    Cells
    Recovery
    Cell Nucleus Shape
    3D printers
    Polyurethanes
    Transition Temperature
    Dynamic mechanical analysis
    Superconducting transition temperature
    Cues
    Technology
    Fabrication

    Cite this

    Hendrikson, Wilhelmus J. ; Rouwkema, Jeroen ; Clementi, Federico ; van Blitterswijk, Clemens ; Farè, Silvia ; Moroni, Lorenzo. / Towards 4D Printed Scaffolds for Tissue Engineering : Exploiting 3D Shape Memory Polymers to Deliver Time-Controlled Stimulus on Cultured Cells. In: Biofabrication. 2017 ; Vol. 9, No. 3.
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    abstract = "Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized by dynamic-mechanical analysis. The thermo-mechanical analysis showed a T g at about 32 °C (T g = T trans), indicating no influence of the fabrication process on the transition temperature. In addition, shape recovery tests showed a good recovery of the permanent shape for both scaffold configurations. When cells were seeded onto the scaffolds in the temporary shape and the permanent shape was recovered, cells were significantly more elongated after shape recovery. Thus, the mechanical stimulus imparted by shape recovery is able to influence the shape of cells and nuclei. The obtained results indicate that a single mechanical stimulus is sufficient to initiate changes in the morphology of adherent cells.",
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    Towards 4D Printed Scaffolds for Tissue Engineering : Exploiting 3D Shape Memory Polymers to Deliver Time-Controlled Stimulus on Cultured Cells. / Hendrikson, Wilhelmus J.; Rouwkema, Jeroen ; Clementi, Federico ; van Blitterswijk, Clemens; Farè, Silvia; Moroni, Lorenzo.

    In: Biofabrication, Vol. 9, No. 3, 031001, 02.08.2017.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Hendrikson, Wilhelmus J.

    AU - Rouwkema, Jeroen

    AU - Clementi, Federico

    AU - van Blitterswijk, Clemens

    AU - Farè, Silvia

    AU - Moroni, Lorenzo

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    AB - Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized by dynamic-mechanical analysis. The thermo-mechanical analysis showed a T g at about 32 °C (T g = T trans), indicating no influence of the fabrication process on the transition temperature. In addition, shape recovery tests showed a good recovery of the permanent shape for both scaffold configurations. When cells were seeded onto the scaffolds in the temporary shape and the permanent shape was recovered, cells were significantly more elongated after shape recovery. Thus, the mechanical stimulus imparted by shape recovery is able to influence the shape of cells and nuclei. The obtained results indicate that a single mechanical stimulus is sufficient to initiate changes in the morphology of adherent cells.

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