Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation

Evelien Kemna, Loes Irene Segerink, R.M. Schoeman, F. Wolbers, I. Vermes, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95% is shown, using inertial focusing in a curved channel.
Original languageUndefined
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011
Place of PublicationWashington
PublisherChemical and Biological Microssytems Society
Pages969-971
Number of pages3
ISBN (Print)978-0-979-8064-4-5
Publication statusPublished - 2 Oct 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011 - Seattle, United States
Duration: 2 Oct 20116 Oct 2011
Conference number: 15
https://microtasconferences.org/microtas2011/

Publication series

Name
PublisherChemical and Biological Microssytems Society
ISSN (Print)1556-5904

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011
Abbreviated titleMicroTAS
CountryUnited States
CitySeattle
Period2/10/116/10/11
Internet address

Keywords

  • METIS-281594
  • Droplets
  • Inertial focusing
  • EWI-20893
  • Cell encapsulation
  • IR-78658
  • Impedance

Cite this

Kemna, E., Segerink, L. I., Schoeman, R. M., Wolbers, F., Vermes, I., & van den Berg, A. (2011). Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011 (pp. 969-971). Washington: Chemical and Biological Microssytems Society.
Kemna, Evelien ; Segerink, Loes Irene ; Schoeman, R.M. ; Wolbers, F. ; Vermes, I. ; van den Berg, Albert. / Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011. Washington : Chemical and Biological Microssytems Society, 2011. pp. 969-971
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title = "Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation",
abstract = "Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95{\%} is shown, using inertial focusing in a curved channel.",
keywords = "METIS-281594, Droplets, Inertial focusing, EWI-20893, Cell encapsulation, IR-78658, Impedance",
author = "Evelien Kemna and Segerink, {Loes Irene} and R.M. Schoeman and F. Wolbers and I. Vermes and {van den Berg}, Albert",
year = "2011",
month = "10",
day = "2",
language = "Undefined",
isbn = "978-0-979-8064-4-5",
publisher = "Chemical and Biological Microssytems Society",
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Kemna, E, Segerink, LI, Schoeman, RM, Wolbers, F, Vermes, I & van den Berg, A 2011, Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011. Chemical and Biological Microssytems Society, Washington, pp. 969-971, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011, Seattle, United States, 2/10/11.

Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation. / Kemna, Evelien; Segerink, Loes Irene; Schoeman, R.M.; Wolbers, F.; Vermes, I.; van den Berg, Albert.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011. Washington : Chemical and Biological Microssytems Society, 2011. p. 969-971.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation

AU - Kemna, Evelien

AU - Segerink, Loes Irene

AU - Schoeman, R.M.

AU - Wolbers, F.

AU - Vermes, I.

AU - van den Berg, Albert

PY - 2011/10/2

Y1 - 2011/10/2

N2 - Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95% is shown, using inertial focusing in a curved channel.

AB - Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95% is shown, using inertial focusing in a curved channel.

KW - METIS-281594

KW - Droplets

KW - Inertial focusing

KW - EWI-20893

KW - Cell encapsulation

KW - IR-78658

KW - Impedance

M3 - Conference contribution

SN - 978-0-979-8064-4-5

SP - 969

EP - 971

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011

PB - Chemical and Biological Microssytems Society

CY - Washington

ER -

Kemna E, Segerink LI, Schoeman RM, Wolbers F, Vermes I, van den Berg A. Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011. Washington: Chemical and Biological Microssytems Society. 2011. p. 969-971