Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation

Evelien Kemna, Loes Irene Segerink, R.M. Schoeman, F. Wolbers, I. Vermes, Albert van den Berg

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95% is shown, using inertial focusing in a curved channel.
    Original languageUndefined
    Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011
    Place of PublicationWashington
    PublisherChemical and Biological Microssytems Society
    Pages969-971
    Number of pages3
    ISBN (Print)978-0-979-8064-4-5
    Publication statusPublished - 2 Oct 2011
    Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011 - Seattle, United States
    Duration: 2 Oct 20116 Oct 2011
    Conference number: 15
    https://microtasconferences.org/microtas2011/

    Publication series

    Name
    PublisherChemical and Biological Microssytems Society
    ISSN (Print)1556-5904

    Conference

    Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011
    Abbreviated titleMicroTAS
    CountryUnited States
    CitySeattle
    Period2/10/116/10/11
    Internet address

    Keywords

    • METIS-281594
    • Droplets
    • Inertial focusing
    • EWI-20893
    • Cell encapsulation
    • IR-78658
    • Impedance

    Cite this

    Kemna, E., Segerink, L. I., Schoeman, R. M., Wolbers, F., Vermes, I., & van den Berg, A. (2011). Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011 (pp. 969-971). Washington: Chemical and Biological Microssytems Society.