Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation

Evelien Kemna; Loes Irene Segerink; R.M. Schoeman; F. Wolbers; I. Vermes; Albert van den Berg

Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation

Evelien Kemna, Loes Irene Segerink, R.M. Schoeman, F. Wolbers, I. Vermes, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95% is shown, using inertial focusing in a curved channel.

Original language	Undefined
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011
Place of Publication	Washington
Publisher	The Chemical and Biological Microsystems Society
Pages	969-971
Number of pages	3
ISBN (Print)	978-0-979-8064-4-5
Publication status	Published - 2 Oct 2011
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011 - Seattle, United States Duration: 2 Oct 2011 → 6 Oct 2011 Conference number: 15 https://microtasconferences.org/microtas2011/

Publication series

Name
Publisher	Chemical and Biological Microssytems Society
ISSN (Print)	1556-5904

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011
Abbreviated title	MicroTAS
Country/Territory	United States
City	Seattle
Period	2/10/11 → 6/10/11
Internet address	https://microtasconferences.org/microtas2011/

Keywords

METIS-281594
Droplets
Inertial focusing
EWI-20893
Cell encapsulation
IR-78658
Impedance

Access to Document

http://eprints.eemcs.utwente.nl/secure2/20893/01/325_0111.pdf

Cite this

Kemna, E., Segerink, L. I., Schoeman, R. M., Wolbers, F., Vermes, I., & van den Berg, A. (2011). Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011 (pp. 969-971). The Chemical and Biological Microsystems Society. http://eprints.eemcs.utwente.nl/secure2/20893/01/325_0111.pdf

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title = "Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation",

abstract = "Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95% is shown, using inertial focusing in a curved channel.",

keywords = "METIS-281594, Droplets, Inertial focusing, EWI-20893, Cell encapsulation, IR-78658, Impedance",

author = "Evelien Kemna and Segerink, {Loes Irene} and R.M. Schoeman and F. Wolbers and I. Vermes and {van den Berg}, Albert",

year = "2011",

month = oct,

day = "2",

language = "Undefined",

isbn = "978-0-979-8064-4-5",

publisher = "The Chemical and Biological Microsystems Society",

pages = "969--971",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

url = "https://microtasconferences.org/microtas2011/",

}

Kemna, E, Segerink, LI, Schoeman, RM, Wolbers, F, Vermes, I & van den Berg, A 2011, Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011. The Chemical and Biological Microsystems Society, Washington, pp. 969-971, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011, Seattle, Washington, United States, 2/10/11. <http://eprints.eemcs.utwente.nl/secure2/20893/01/325_0111.pdf>

Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation. / Kemna, Evelien; Segerink, Loes Irene; Schoeman, R.M. et al.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011. Washington: The Chemical and Biological Microsystems Society, 2011. p. 969-971.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Towards a high-throughput electrofusion platform using droplets: cell detection and deterministic encapsulation

AU - Kemna, Evelien

AU - Segerink, Loes Irene

AU - Schoeman, R.M.

AU - Wolbers, F.

AU - Vermes, I.

AU - van den Berg, Albert

N1 - Conference code: 15

PY - 2011/10/2

Y1 - 2011/10/2

N2 - Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95% is shown, using inertial focusing in a curved channel.

AB - Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95% is shown, using inertial focusing in a curved channel.

KW - METIS-281594

KW - Droplets

KW - Inertial focusing

KW - EWI-20893

KW - Cell encapsulation

KW - IR-78658

KW - Impedance

M3 - Conference contribution

SN - 978-0-979-8064-4-5

SP - 969

EP - 971

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011

PB - The Chemical and Biological Microsystems Society

CY - Washington

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -