Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylation-sensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels for multiplexed “barcode” sensing of methylation markers.
|Publication status||Published - 14 Oct 2021|
|Event||25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States|
Duration: 10 Oct 2021 → 14 Oct 2021
Conference number: 25
|Conference||25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021|
|Abbreviated title||MicroTAS 2021|
|Period||10/10/21 → 14/10/21|