Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites.

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Abstract

Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylationsensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels for
multiplexed “barcode” sensing of methylation markers.
Original languageEnglish
Title of host publication25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publication 10 - 14 October 2021, Palm Springs, CA, USA
Pages741-742
Publication statusPublished - 11 Oct 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States
Duration: 10 Oct 202114 Oct 2021
Conference number: 25
https://microtas2021.org/

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021
Abbreviated titleMicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs
Period10/10/2114/10/21
Internet address

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