Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites.

Jeanne Elisabeth van Dongen; Johanna T.W. Berendsen; Jan C.T. Eijkel; Loes Segerink

Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites.

Jeanne Elisabeth van Dongen^*, Johanna T.W. Berendsen, Jan C.T. Eijkel, Loes Segerink

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylationsensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels for
multiplexed “barcode” sensing of methylation markers.

Original language	English
Title of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publication	10 - 14 October 2021, Palm Springs, CA, USA
Pages	741-742
Publication status	Published - 11 Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States Duration: 10 Oct 2021 → 14 Oct 2021 Conference number: 25 https://microtas2021.org/

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021
Abbreviated title	MicroTAS 2021
Country/Territory	United States
City	Palm Springs
Period	10/10/21 → 14/10/21
Internet address	https://microtas2021.org/

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van Dongen, Jeanne Elisabeth ; Berendsen, Johanna T.W. ; Eijkel, Jan C.T. et al. / Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites. 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA. 2021. pp. 741-742

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title = "Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites.",

abstract = "Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylationsensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels formultiplexed “barcode” sensing of methylation markers.",

author = "{van Dongen}, {Jeanne Elisabeth} and Berendsen, {Johanna T.W.} and Eijkel, {Jan C.T.} and Loes Segerink",

year = "2021",

month = oct,

day = "11",

language = "English",

pages = "741--742",

booktitle = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

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}

van Dongen, JE , Berendsen, JTW , Eijkel, JCT & Segerink, L 2021, Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites. in 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA. pp. 741-742, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, Palm Springs, California, United States, 10/10/21.

Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites. / van Dongen, Jeanne Elisabeth ; Berendsen, Johanna T.W.; Eijkel, Jan C.T. et al.
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA. 2021. p. 741-742.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites.

AU - van Dongen, Jeanne Elisabeth

AU - Berendsen, Johanna T.W.

AU - Eijkel, Jan C.T.

AU - Segerink, Loes

N1 - Conference code: 25

PY - 2021/10/11

Y1 - 2021/10/11

N2 - Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylationsensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels formultiplexed “barcode” sensing of methylation markers.

AB - Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylationsensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels formultiplexed “barcode” sensing of methylation markers.

M3 - Conference contribution

SP - 741

EP - 742

BT - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Towards a multiplexed barcode CRISPR/Cas12a-Assisted platform for the identification and quantification of singele CpG Methylation Sites.

Abstract

Conference

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