Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites

Jeanne Elisabeth van Dongen; Johanna T.W. Berendsen; Jan C.T. Eijkel; Loes Segerink

Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites

Jeanne Elisabeth van Dongen^*
, Johanna T.W. Berendsen
, Jan C.T. Eijkel
, Loes Segerink

^*Corresponding author for this work

Research output: Contribution to conference › Poster › Academic

Abstract

Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylation-sensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels for multiplexed “barcode” sensing of methylation markers.

Original language	English
Publication status	Published - 14 Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States Duration: 10 Oct 2021 → 14 Oct 2021 Conference number: 25 https://microtas2021.org/

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021
Abbreviated title	MicroTAS 2021
Country/Territory	United States
City	Palm Springs
Period	10/10/21 → 14/10/21
Internet address	https://microtas2021.org/

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van Dongen, J. E., Berendsen, J. T. W., Eijkel, J. C. T., & Segerink, L. (2021). Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites. Poster session presented at 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, Palm Springs, California, United States.

van Dongen, Jeanne Elisabeth ; Berendsen, Johanna T.W. ; Eijkel, Jan C.T. et al. / Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites. Poster session presented at 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, Palm Springs, California, United States.

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title = "Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites",

abstract = "Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylation-sensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels for multiplexed “barcode” sensing of methylation markers. ",

author = "\{van Dongen\}, \{Jeanne Elisabeth\} and Berendsen, \{Johanna T.W.\} and Eijkel, \{Jan C.T.\} and Loes Segerink",

year = "2021",

month = oct,

day = "14",

language = "English",

note = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

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Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites. / van Dongen, Jeanne Elisabeth; Berendsen, Johanna T.W.; Eijkel, Jan C.T. et al.
2021. Poster session presented at 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, Palm Springs, California, United States.

Research output: Contribution to conference › Poster › Academic

TY - CONF

T1 - Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites

AU - van Dongen, Jeanne Elisabeth

AU - Berendsen, Johanna T.W.

AU - Eijkel, Jan C.T.

AU - Segerink, Loes

N1 - Conference code: 25

PY - 2021/10/14

Y1 - 2021/10/14

N2 - Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylation-sensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels for multiplexed “barcode” sensing of methylation markers.

AB - Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylation-sensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels for multiplexed “barcode” sensing of methylation markers.

M3 - Poster

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites

Abstract

Conference

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