Towards microfluidic sperm refinement : impedance-based analysis and sorting of sperm cells

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)
4 Downloads (Pure)

Abstract

The use of high quality semen for artificial insemination in the livestock industry is essential for successful outcome. Insemination using semen with a high number of sperm cells containing morphological defects has a negative impact on fertilization outcome. Therefore, semen with a high number of these abnormal cells is discarded in order to maintain high fertilization potential, resulting in the loss of a large number of morphologically normal sperm cells (up to 70-80% of original sample). A commonly occurring morphological sperm anomaly is the cytoplasmic droplet on the sperm flagella. Currently, no techniques are available to extract morphologically normal sperm cells from rejected samples. Therefore, we aim to develop a microfluidic setup which is able to detect and sort morphologically normal sperm cells label-free and non-invasively. In a proof-of-concept experiment, differential impedance measurements were used to detect the presence of cytoplasmic droplets on sperm flagella, which was quantified by calculating the area under the curve (AUC) of the corresponding impedance peaks. A receiver operating characteristic curve of this electrical analysis method showed the good predictive power of this analysis method (AUC value of 0.85). Furthermore, we developed a label-free cell sorting system using LabVIEW, which is capable of sorting sperm cells based on impedance. In a proof-of-concept experiment, sperm cells and 3 mu m beads were sorted label-free and non-invasively using impedance detection and dielectrophoresis sorting. These experiments present our first attempt to perform sperm refinement using microfluidic technology.
Original languageUndefined
Pages (from-to)1514-1522
Number of pages9
JournalLab on a chip
Volume16
Issue number8
DOIs
Publication statusPublished - 2016

Keywords

  • EWI-27095
  • METIS-318468
  • IR-101016

Cite this

@article{e9845be3449341c481e537079790f87a,
title = "Towards microfluidic sperm refinement : impedance-based analysis and sorting of sperm cells",
abstract = "The use of high quality semen for artificial insemination in the livestock industry is essential for successful outcome. Insemination using semen with a high number of sperm cells containing morphological defects has a negative impact on fertilization outcome. Therefore, semen with a high number of these abnormal cells is discarded in order to maintain high fertilization potential, resulting in the loss of a large number of morphologically normal sperm cells (up to 70-80{\%} of original sample). A commonly occurring morphological sperm anomaly is the cytoplasmic droplet on the sperm flagella. Currently, no techniques are available to extract morphologically normal sperm cells from rejected samples. Therefore, we aim to develop a microfluidic setup which is able to detect and sort morphologically normal sperm cells label-free and non-invasively. In a proof-of-concept experiment, differential impedance measurements were used to detect the presence of cytoplasmic droplets on sperm flagella, which was quantified by calculating the area under the curve (AUC) of the corresponding impedance peaks. A receiver operating characteristic curve of this electrical analysis method showed the good predictive power of this analysis method (AUC value of 0.85). Furthermore, we developed a label-free cell sorting system using LabVIEW, which is capable of sorting sperm cells based on impedance. In a proof-of-concept experiment, sperm cells and 3 mu m beads were sorted label-free and non-invasively using impedance detection and dielectrophoresis sorting. These experiments present our first attempt to perform sperm refinement using microfluidic technology.",
keywords = "EWI-27095, METIS-318468, IR-101016",
author = "{de Wagenaar}, B. and Stefan Dekker and {de Boer}, {Hans L.} and Bomer, {Johan G.} and Wouter Olthuis and {van den Berg}, Albert and Segerink, {Loes Irene}",
note = "eemcs-eprint-27095",
year = "2016",
doi = "10.1039/c6lc00256k",
language = "Undefined",
volume = "16",
pages = "1514--1522",
journal = "Lab on a chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "8",

}

Towards microfluidic sperm refinement : impedance-based analysis and sorting of sperm cells. / de Wagenaar, B.; Dekker, Stefan; de Boer, Hans L.; Bomer, Johan G.; Olthuis, Wouter; van den Berg, Albert; Segerink, Loes Irene.

In: Lab on a chip, Vol. 16, No. 8, 2016, p. 1514-1522.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Towards microfluidic sperm refinement : impedance-based analysis and sorting of sperm cells

AU - de Wagenaar, B.

AU - Dekker, Stefan

AU - de Boer, Hans L.

AU - Bomer, Johan G.

AU - Olthuis, Wouter

AU - van den Berg, Albert

AU - Segerink, Loes Irene

N1 - eemcs-eprint-27095

PY - 2016

Y1 - 2016

N2 - The use of high quality semen for artificial insemination in the livestock industry is essential for successful outcome. Insemination using semen with a high number of sperm cells containing morphological defects has a negative impact on fertilization outcome. Therefore, semen with a high number of these abnormal cells is discarded in order to maintain high fertilization potential, resulting in the loss of a large number of morphologically normal sperm cells (up to 70-80% of original sample). A commonly occurring morphological sperm anomaly is the cytoplasmic droplet on the sperm flagella. Currently, no techniques are available to extract morphologically normal sperm cells from rejected samples. Therefore, we aim to develop a microfluidic setup which is able to detect and sort morphologically normal sperm cells label-free and non-invasively. In a proof-of-concept experiment, differential impedance measurements were used to detect the presence of cytoplasmic droplets on sperm flagella, which was quantified by calculating the area under the curve (AUC) of the corresponding impedance peaks. A receiver operating characteristic curve of this electrical analysis method showed the good predictive power of this analysis method (AUC value of 0.85). Furthermore, we developed a label-free cell sorting system using LabVIEW, which is capable of sorting sperm cells based on impedance. In a proof-of-concept experiment, sperm cells and 3 mu m beads were sorted label-free and non-invasively using impedance detection and dielectrophoresis sorting. These experiments present our first attempt to perform sperm refinement using microfluidic technology.

AB - The use of high quality semen for artificial insemination in the livestock industry is essential for successful outcome. Insemination using semen with a high number of sperm cells containing morphological defects has a negative impact on fertilization outcome. Therefore, semen with a high number of these abnormal cells is discarded in order to maintain high fertilization potential, resulting in the loss of a large number of morphologically normal sperm cells (up to 70-80% of original sample). A commonly occurring morphological sperm anomaly is the cytoplasmic droplet on the sperm flagella. Currently, no techniques are available to extract morphologically normal sperm cells from rejected samples. Therefore, we aim to develop a microfluidic setup which is able to detect and sort morphologically normal sperm cells label-free and non-invasively. In a proof-of-concept experiment, differential impedance measurements were used to detect the presence of cytoplasmic droplets on sperm flagella, which was quantified by calculating the area under the curve (AUC) of the corresponding impedance peaks. A receiver operating characteristic curve of this electrical analysis method showed the good predictive power of this analysis method (AUC value of 0.85). Furthermore, we developed a label-free cell sorting system using LabVIEW, which is capable of sorting sperm cells based on impedance. In a proof-of-concept experiment, sperm cells and 3 mu m beads were sorted label-free and non-invasively using impedance detection and dielectrophoresis sorting. These experiments present our first attempt to perform sperm refinement using microfluidic technology.

KW - EWI-27095

KW - METIS-318468

KW - IR-101016

U2 - 10.1039/c6lc00256k

DO - 10.1039/c6lc00256k

M3 - Article

VL - 16

SP - 1514

EP - 1522

JO - Lab on a chip

JF - Lab on a chip

SN - 1473-0197

IS - 8

ER -