TY - JOUR
T1 - Towards single-cell bioprinting
T2 - micropatterning tools for organ-on-chip development
AU - Bosmans, Cécile
AU - Ginés Rodriguez, Núria
AU - Karperien, Marcel
AU - Malda, Jos
AU - Moreira Teixeira, Liliana
AU - Levato, Riccardo
AU - Leijten, Jeroen
N1 - Publisher Copyright:
© 2023 The Author(s)
PY - 2024/6
Y1 - 2024/6
N2 - Organs-on-chips (OoCs) hold promise to engineer progressively more human-relevant in vitro models for pharmaceutical purposes. Recent developments have delivered increasingly sophisticated designs, yet OoCs still lack in reproducing the inner tissue physiology required to fully resemble the native human body. This review emphasizes the need to include microarchitectural and microstructural features, and discusses promising avenues to incorporate well-defined microarchitectures down to the single-cell level. We highlight how their integration will significantly contribute to the advancement of the field towards highly organized structural and hierarchical tissues-on-chip. We discuss the combination of state-of-the-art micropatterning technologies to achieve OoCs resembling human-intrinsic complexity. It is anticipated that these innovations will yield significant advances in realization of the next generation of OoC models.
AB - Organs-on-chips (OoCs) hold promise to engineer progressively more human-relevant in vitro models for pharmaceutical purposes. Recent developments have delivered increasingly sophisticated designs, yet OoCs still lack in reproducing the inner tissue physiology required to fully resemble the native human body. This review emphasizes the need to include microarchitectural and microstructural features, and discusses promising avenues to incorporate well-defined microarchitectures down to the single-cell level. We highlight how their integration will significantly contribute to the advancement of the field towards highly organized structural and hierarchical tissues-on-chip. We discuss the combination of state-of-the-art micropatterning technologies to achieve OoCs resembling human-intrinsic complexity. It is anticipated that these innovations will yield significant advances in realization of the next generation of OoC models.
KW - biofabrication
KW - disease models
KW - laser-induced forward transfer
KW - microfluidics
KW - microphysiological systems
KW - UT-Hybrid-D
UR - http://www.scopus.com/inward/record.url?scp=85184059497&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2023.11.014
DO - 10.1016/j.tibtech.2023.11.014
M3 - Review article
C2 - 38310021
AN - SCOPUS:85184059497
SN - 0167-7799
VL - 42
SP - 739
EP - 759
JO - Trends in biotechnology
JF - Trends in biotechnology
IS - 6
ER -