Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues

D. Rana*, N. Salehi Nik (Contributor), J. Rouwkema (Contributor)

*Corresponding author for this work

    Research output: Contribution to conferenceAbstract

    Abstract

    Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.
    Original languageEnglish
    Publication statusPublished - 20 Jun 2019
    EventBiofabrication & Biomanufacturing Europe 2019 : SelectBio - Rotterdam Marriot Hotel, Rotterdam, Netherlands
    Duration: 20 Jun 201921 Jun 2019
    http://selectbiosciences.com/conferences/index.aspx?conf=BIOFABEU2019

    Conference

    ConferenceBiofabrication & Biomanufacturing Europe 2019 
    Abbreviated titleSelectBio
    CountryNetherlands
    CityRotterdam
    Period20/06/1921/06/19
    Internet address

    Fingerprint

    Hydrogels
    Blood Vessels
    Vascular Endothelial Growth Factor A
    Human Umbilical Vein Endothelial Cells
    Coculture Techniques
    Mesenchymal Stromal Cells
    Gelatin
    Polymerization
    Cell Survival
    Intercellular Signaling Peptides and Proteins
    Enzyme-Linked Immunosorbent Assay
    Control Groups

    Keywords

    • Vascularization
    • Tissue engineering

    Cite this

    Rana, D., Salehi Nik, N., & Rouwkema, J. (2019). Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues. Abstract from Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands.
    Rana, D. ; Salehi Nik, N. ; Rouwkema, J. / Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues. Abstract from Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands.
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    abstract = "Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.",
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    Rana, D, Salehi Nik, N & Rouwkema, J 2019, 'Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues' Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands, 20/06/19 - 21/06/19, .

    Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues. / Rana, D.; Salehi Nik, N. (Contributor); Rouwkema, J. (Contributor).

    2019. Abstract from Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands.

    Research output: Contribution to conferenceAbstract

    TY - CONF

    T1 - Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues

    AU - Rana, D.

    A2 - Salehi Nik, N.

    A2 - Rouwkema, J.

    PY - 2019/6/20

    Y1 - 2019/6/20

    N2 - Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.

    AB - Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.

    KW - Vascularization

    KW - Tissue engineering

    M3 - Abstract

    ER -

    Rana D, Salehi Nik N, Rouwkema J. Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues. 2019. Abstract from Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands.