Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues

D. Rana, N. Salehi Nik (Contributor), J. Rouwkema (Contributor)

Research output: Contribution to conferenceAbstractAcademic

Abstract

Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.
Original languageEnglish
Publication statusPublished - 20 Jun 2019
EventBiofabrication & Biomanufacturing Europe 2019 : SelectBio - Rotterdam Marriot Hotel, Rotterdam, Netherlands
Duration: 20 Jun 201921 Jun 2019
http://selectbiosciences.com/conferences/index.aspx?conf=BIOFABEU2019

Conference

ConferenceBiofabrication & Biomanufacturing Europe 2019 
Abbreviated titleSelectBio
CountryNetherlands
CityRotterdam
Period20/06/1921/06/19
Internet address

Fingerprint

Hydrogels
Blood Vessels
Vascular Endothelial Growth Factor A
Human Umbilical Vein Endothelial Cells
Coculture Techniques
Mesenchymal Stromal Cells
Gelatin
Polymerization
Cell Survival
Intercellular Signaling Peptides and Proteins
Enzyme-Linked Immunosorbent Assay
Control Groups

Keywords

  • Vascularization
  • Tissue engineering

Cite this

Rana, D., Salehi Nik, N., & Rouwkema, J. (2019). Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues. Abstract from Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands.
Rana, D. ; Salehi Nik, N. ; Rouwkema, J. / Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues. Abstract from Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands.
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abstract = "Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.",
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Rana, D, Salehi Nik, N & Rouwkema, J 2019, 'Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues' Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands, 20/06/19 - 21/06/19, .

Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues. / Rana, D.; Salehi Nik, N. (Contributor); Rouwkema, J. (Contributor).

2019. Abstract from Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands.

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues

AU - Rana, D.

A2 - Salehi Nik, N.

A2 - Rouwkema, J.

PY - 2019/6/20

Y1 - 2019/6/20

N2 - Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.

AB - Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.

KW - Vascularization

KW - Tissue engineering

M3 - Abstract

ER -

Rana D, Salehi Nik N, Rouwkema J. Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues. 2019. Abstract from Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands.