Abstract
| Original language | English |
|---|---|
| Publication status | Published - 20 Jun 2019 |
| Event | Biofabrication & Biomanufacturing Europe 2019 : SelectBio - Rotterdam Marriot Hotel, Rotterdam, Netherlands Duration: 20 Jun 2019 → 21 Jun 2019 http://selectbiosciences.com/conferences/index.aspx?conf=BIOFABEU2019 |
Conference
| Conference | Biofabrication & Biomanufacturing Europe 2019 |
|---|---|
| Abbreviated title | SelectBio |
| Country | Netherlands |
| City | Rotterdam |
| Period | 20/06/19 → 21/06/19 |
| Internet address |
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Keywords
- Vascularization
- Tissue engineering
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Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues. / Rana, D.; Salehi Nik, N. (Contributor); Rouwkema, J. (Contributor).
2019. Poster session presented at Biofabrication & Biomanufacturing Europe 2019 , Rotterdam, Netherlands.Research output: Contribution to conference › Poster › Academic
TY - CONF
T1 - Towards Spatiotemporally Controlled Vascular Network Formation within Engineered Tissues
AU - Rana, D.
A2 - Salehi Nik, N.
A2 - Rouwkema, J.
PY - 2019/6/20
Y1 - 2019/6/20
N2 - Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.
AB - Spatiotemporally controlled vascular network formation is of great importance for facilitating vascularization within engineered tissues. Therefore, we have designed aptamer-functionalized hydrogels to evaluate their potential for growth factor sequestering, controlled release profiles and study their effect on vascular network formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). The aptamer-functionalized hydrogels were prepared via photo-polymerization of gelatin methacryloyl and acrydite modified vascular endothelial growth factor specific aptamers along with irgacure 2959. The physicochemical properties analysis of these aptamer-functionalized hydrogels showed higher aptamer retaining capacity within the acrydite modified hydrogels than the control group for as long as 14 days at 37 °C. Furthermore, the VEGF ELISA results confirmed the triggered VEGF release from the aptamer-functionalized hydrogels in the presence of complementary sequence; thus showing on-demand release. In co-culture conditions, the aptamer-functionalized hydrogels supported cell viability and the formation of vascular networks with HUVECs and MSCs within the hydrogels for up to 8 days. These initial results confirmed the bioactivity of the bound VEGF molecules after their loading within the aptamer functionalized hydrogels; and their ability to control vascular network formation spatiotemporally.
KW - Vascularization
KW - Tissue engineering
M3 - Poster
ER -