Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells

FANTOM Consortium

    Research output: Contribution to journalArticleAcademicpeer-review

    288 Citations (Scopus)

    Abstract

    Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets of genes has not been previously studied en masse. Exploiting the fact that active promoters and enhancers are transcribed, we simultaneously measured their activity in 19 human and 14 mouse time courses covering a wide range of cell types and biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated the earliest responses. Binding sites for key lineage transcription factors were simultaneously overrepresented in enhancers and promoters active in each cellular system. Our data support a highly generalizable model in which enhancer transcription is the earliest event in successive waves of transcriptional change during cellular differentiation or activation.

    Original languageEnglish
    Pages (from-to)1010-1014
    Number of pages5
    JournalScience
    Volume347
    Issue number6225
    DOIs
    Publication statusPublished - 27 Feb 2015

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