Transfer printing of DNA by "click" chemistry

D.I. Rozkiewicz, Johannes Gierlich, Glenn A. Burley, Katrin Gutschmiedl, Thomas Carell, B.J. Ravoo, David Reinhoudt

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    83 Citations (Scopus)

    Abstract

    This paper describes a straightforward procedure to immobilize oligonucleotides on glass substrates in well-defined micropatterns by microcontact printing with a dendrimer-modified stamp. The oligonucleotides are efficiently immobilized by click chemistry induced by microcontact printing. Acetylene-modified oligonucleotides were treated with an azide-terminated glass slide under the confinement of the dendrimer-modified stamp, without the use of a CuI catalyst. The immobilization is an irreversible, covalent, and one-step reaction that results in stable attachment of the oligonucleotides. Oligonucleotides with the acetylene-modification at the 5 terminus hybridize selectively with full-length, complementary targets. Strands with more than one acetylene linker do not hybridize with complementary strands.
    Original languageUndefined
    Pages (from-to)1997-2002
    Number of pages6
    JournalChemBioChem
    Volume8
    Issue number16
    DOIs
    Publication statusPublished - 2007

    Keywords

    • IR-59256
    • METIS-243315

    Cite this

    Rozkiewicz, D. I., Gierlich, J., Burley, G. A., Gutschmiedl, K., Carell, T., Ravoo, B. J., & Reinhoudt, D. (2007). Transfer printing of DNA by "click" chemistry. ChemBioChem, 8(16), 1997-2002. https://doi.org/10.1002/cbic.200700402