Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

R.B.M. Schasfoort (Corresponding Author), Fekri Abali, Ivan Stojanovic, Gestur Vidarsson, Leon Terstappen

Research output: Contribution to journalReview articleAcademicpeer-review

3 Citations (Scopus)
5 Downloads (Pure)

Abstract

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.
Original languageEnglish
Article number102
Number of pages11
JournalBiosensors (Switserland)
Volume8
Issue number4
DOIs
Publication statusPublished - 30 Oct 2018

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
Labels
Cells
Imaging techniques
Research Personnel
Availability
Sensors

Keywords

  • cytometry
  • surface plasmon resonance
  • single-cell secretion
  • SPR imaging

Cite this

@article{ea746420316a4445a5e7f30a57c7e9ee,
title = "Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters",
abstract = "SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.",
keywords = "cytometry, surface plasmon resonance, single-cell secretion, SPR imaging",
author = "R.B.M. Schasfoort and Fekri Abali and Ivan Stojanovic and Gestur Vidarsson and Leon Terstappen",
year = "2018",
month = "10",
day = "30",
doi = "10.3390/bios8040102",
language = "English",
volume = "8",
journal = "Biosensors (Switserland)",
issn = "2079-6374",
publisher = "Multidisciplinary Digital Publishing Institute",
number = "4",

}

Trends in SPR Cytometry : Advances in Label-Free Detection of Cell Parameters. / Schasfoort, R.B.M. (Corresponding Author); Abali, Fekri ; Stojanovic, Ivan ; Vidarsson, Gestur; Terstappen, Leon.

In: Biosensors (Switserland), Vol. 8, No. 4, 102, 30.10.2018.

Research output: Contribution to journalReview articleAcademicpeer-review

TY - JOUR

T1 - Trends in SPR Cytometry

T2 - Advances in Label-Free Detection of Cell Parameters

AU - Schasfoort, R.B.M.

AU - Abali, Fekri

AU - Stojanovic, Ivan

AU - Vidarsson, Gestur

AU - Terstappen, Leon

PY - 2018/10/30

Y1 - 2018/10/30

N2 - SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

AB - SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

KW - cytometry

KW - surface plasmon resonance

KW - single-cell secretion

KW - SPR imaging

UR - http://www.scopus.com/inward/record.url?scp=85055856378&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/trends-spr-cytometry-advances-labelfree-detection-cell-parameters

U2 - 10.3390/bios8040102

DO - 10.3390/bios8040102

M3 - Review article

VL - 8

JO - Biosensors (Switserland)

JF - Biosensors (Switserland)

SN - 2079-6374

IS - 4

M1 - 102

ER -