Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids

Jean-Baptiste Blondé, Dwi Priwitaningrum, Jai Prakash, Séverine le Gac

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Here, we report a breast tumour-on-a-chip platform to evaluate the penetration of nanomedicines in 3D tumour spheroids, as a function of the tumour spheroid cellular composition. Mouse multicellular tumour mono-culture spheroids and cancer cell-fibroblast co-culture spheroids were exposed to silica nanoparticles (NPs) (30 or 100nm) or liposomes (100nm) at various flowrates, mimicking the shear stress levels found in vivo. Results revealed that the NP penetration was influenced by the shear stress, and that the presence of fibroblasts in the tumour spheroids greatly reduced the penetration depth of the nanoparticles.
Original languageEnglish
Title of host publicationMicroTAS 2018
Subtitle of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherThe Chemical and Biological Microsystems Society
Number of pages2
ISBN (Print)978-0-578-40530-8
Publication statusPublished - 13 Nov 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, Province of China
Duration: 10 Nov 201815 Nov 2018
Conference number: 22
https://cbmsociety.org/conferences/microtas2018/

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated titleMicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period10/11/1815/11/18
Internet address

Fingerprint

Coculture Techniques
Nanoparticles
Neoplasms
Fibroblasts
Cellular Spheroids
Nanomedicine
Liposomes
Silicon Dioxide
Breast Neoplasms

Keywords

  • Microfluidics
  • Tumour Spheroid
  • Nanomedicine

Cite this

Blondé, J-B., Priwitaningrum, D., Prakash, J., & le Gac, S. (2018). Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids. In MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences The Chemical and Biological Microsystems Society.
Blondé, Jean-Baptiste ; Priwitaningrum, Dwi ; Prakash, Jai ; le Gac, Séverine . / Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids. MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, 2018.
@inproceedings{58e8542a085b453ea232567570ffb7d8,
title = "Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids",
abstract = "Here, we report a breast tumour-on-a-chip platform to evaluate the penetration of nanomedicines in 3D tumour spheroids, as a function of the tumour spheroid cellular composition. Mouse multicellular tumour mono-culture spheroids and cancer cell-fibroblast co-culture spheroids were exposed to silica nanoparticles (NPs) (30 or 100nm) or liposomes (100nm) at various flowrates, mimicking the shear stress levels found in vivo. Results revealed that the NP penetration was influenced by the shear stress, and that the presence of fibroblasts in the tumour spheroids greatly reduced the penetration depth of the nanoparticles.",
keywords = "Microfluidics, Tumour Spheroid, Nanomedicine",
author = "Jean-Baptiste Blond{\'e} and Dwi Priwitaningrum and Jai Prakash and {le Gac}, S{\'e}verine",
year = "2018",
month = "11",
day = "13",
language = "English",
isbn = "978-0-578-40530-8",
booktitle = "MicroTAS 2018",
publisher = "The Chemical and Biological Microsystems Society",

}

Blondé, J-B, Priwitaningrum, D, Prakash, J & le Gac, S 2018, Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids. in MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, Kaohsiung, Taiwan, Province of China, 10/11/18.

Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids. / Blondé, Jean-Baptiste; Priwitaningrum, Dwi; Prakash, Jai; le Gac, Séverine .

MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, 2018.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids

AU - Blondé, Jean-Baptiste

AU - Priwitaningrum, Dwi

AU - Prakash, Jai

AU - le Gac, Séverine

PY - 2018/11/13

Y1 - 2018/11/13

N2 - Here, we report a breast tumour-on-a-chip platform to evaluate the penetration of nanomedicines in 3D tumour spheroids, as a function of the tumour spheroid cellular composition. Mouse multicellular tumour mono-culture spheroids and cancer cell-fibroblast co-culture spheroids were exposed to silica nanoparticles (NPs) (30 or 100nm) or liposomes (100nm) at various flowrates, mimicking the shear stress levels found in vivo. Results revealed that the NP penetration was influenced by the shear stress, and that the presence of fibroblasts in the tumour spheroids greatly reduced the penetration depth of the nanoparticles.

AB - Here, we report a breast tumour-on-a-chip platform to evaluate the penetration of nanomedicines in 3D tumour spheroids, as a function of the tumour spheroid cellular composition. Mouse multicellular tumour mono-culture spheroids and cancer cell-fibroblast co-culture spheroids were exposed to silica nanoparticles (NPs) (30 or 100nm) or liposomes (100nm) at various flowrates, mimicking the shear stress levels found in vivo. Results revealed that the NP penetration was influenced by the shear stress, and that the presence of fibroblasts in the tumour spheroids greatly reduced the penetration depth of the nanoparticles.

KW - Microfluidics

KW - Tumour Spheroid

KW - Nanomedicine

M3 - Conference contribution

SN - 978-0-578-40530-8

BT - MicroTAS 2018

PB - The Chemical and Biological Microsystems Society

ER -

Blondé J-B, Priwitaningrum D, Prakash J, le Gac S. Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids. In MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society. 2018