Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids

Jean-Baptiste Blondé; Dwi Priwitaningrum; Jai Prakash; Séverine  le Gac

Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids

Jean-Baptiste Blondé
, Dwi Priwitaningrum
, Jai Prakash
, Séverine le Gac

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Here, we report a breast tumour-on-a-chip platform to evaluate the penetration of nanomedicines in 3D tumour spheroids, as a function of the tumour spheroid cellular composition. Mouse multicellular tumour mono-culture spheroids and cancer cell-fibroblast co-culture spheroids were exposed to silica nanoparticles (NPs) (30 or 100nm) or liposomes (100nm) at various flowrates, mimicking the shear stress levels found in vivo. Results revealed that the NP penetration was influenced by the shear stress, and that the presence of fibroblasts in the tumour spheroids greatly reduced the penetration depth of the nanoparticles.

Original language	English
Title of host publication	MicroTAS 2018
Subtitle of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	Fan-Gang Tseng, Gwo-Bin Lee
Publisher	The Chemical and Biological Microsystems Society
Pages	1959-1960
Number of pages	2
ISBN (Electronic)	978-1-510-89757-1
ISBN (Print)	978-0-578-40530-8
Publication status	Published - 13 Nov 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan Duration: 10 Nov 2018 → 15 Nov 2018 Conference number: 22 https://cbmsociety.org/conferences/microtas2018/

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated title	MicroTAS 2018
Country/Territory	Taiwan
City	Kaohsiung
Period	10/11/18 → 15/11/18
Internet address	https://cbmsociety.org/conferences/microtas2018/

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Microfluidics
Tumour Spheroid
Nanomedicine

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Blondé, J.-B., Priwitaningrum, D., Prakash, J., & le Gac, S. (2018). Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids. In F.-G. Tseng, & G.-B. Lee (Eds.), MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1959-1960). The Chemical and Biological Microsystems Society.

Blondé, Jean-Baptiste ; Priwitaningrum, Dwi ; Prakash, Jai et al. / Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids. MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / Fan-Gang Tseng ; Gwo-Bin Lee. The Chemical and Biological Microsystems Society, 2018. pp. 1959-1960

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title = "Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids",

abstract = "Here, we report a breast tumour-on-a-chip platform to evaluate the penetration of nanomedicines in 3D tumour spheroids, as a function of the tumour spheroid cellular composition. Mouse multicellular tumour mono-culture spheroids and cancer cell-fibroblast co-culture spheroids were exposed to silica nanoparticles (NPs) (30 or 100nm) or liposomes (100nm) at various flowrates, mimicking the shear stress levels found in vivo. Results revealed that the NP penetration was influenced by the shear stress, and that the presence of fibroblasts in the tumour spheroids greatly reduced the penetration depth of the nanoparticles.",

keywords = "Microfluidics, Tumour Spheroid, Nanomedicine",

author = "Jean-Baptiste Blond{\'e} and Dwi Priwitaningrum and Jai Prakash and \{le Gac\}, S{\'e}verine",

year = "2018",

month = nov,

day = "13",

language = "English",

isbn = "978-0-578-40530-8",

pages = "1959--1960",

editor = "Fan-Gang Tseng and Gwo-Bin Lee",

booktitle = "MicroTAS 2018",

publisher = "The Chemical and Biological Microsystems Society",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, MicroTAS 2018 ; Conference date: 10-11-2018 Through 15-11-2018",

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}

Blondé, J-B, Priwitaningrum, D, Prakash, J & le Gac, S 2018, Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids. in F-G Tseng & G-B Lee (eds), MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, pp. 1959-1960, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, Kaohsiung, Taiwan, 10/11/18.

Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids. / Blondé, Jean-Baptiste; Priwitaningrum, Dwi; Prakash, Jai et al.
MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / Fan-Gang Tseng; Gwo-Bin Lee. The Chemical and Biological Microsystems Society, 2018. p. 1959-1960.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids

AU - Blondé, Jean-Baptiste

AU - Priwitaningrum, Dwi

AU - Prakash, Jai

AU - le Gac, Séverine

N1 - Conference code: 22

PY - 2018/11/13

Y1 - 2018/11/13

N2 - Here, we report a breast tumour-on-a-chip platform to evaluate the penetration of nanomedicines in 3D tumour spheroids, as a function of the tumour spheroid cellular composition. Mouse multicellular tumour mono-culture spheroids and cancer cell-fibroblast co-culture spheroids were exposed to silica nanoparticles (NPs) (30 or 100nm) or liposomes (100nm) at various flowrates, mimicking the shear stress levels found in vivo. Results revealed that the NP penetration was influenced by the shear stress, and that the presence of fibroblasts in the tumour spheroids greatly reduced the penetration depth of the nanoparticles.

AB - Here, we report a breast tumour-on-a-chip platform to evaluate the penetration of nanomedicines in 3D tumour spheroids, as a function of the tumour spheroid cellular composition. Mouse multicellular tumour mono-culture spheroids and cancer cell-fibroblast co-culture spheroids were exposed to silica nanoparticles (NPs) (30 or 100nm) or liposomes (100nm) at various flowrates, mimicking the shear stress levels found in vivo. Results revealed that the NP penetration was influenced by the shear stress, and that the presence of fibroblasts in the tumour spheroids greatly reduced the penetration depth of the nanoparticles.

KW - Microfluidics

KW - Tumour Spheroid

KW - Nanomedicine

M3 - Conference contribution

SN - 978-0-578-40530-8

SP - 1959

EP - 1960

BT - MicroTAS 2018

A2 - Tseng, Fan-Gang

A2 - Lee, Gwo-Bin

PB - The Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018

Y2 - 10 November 2018 through 15 November 2018

ER -

Tumour-on-a-chip platform to evaluate nanoparticles penetration in 3D co-culture tumour spheroids

Abstract

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Keywords

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