Tunable and Compartmentalized Multimaterial Bioprinting for Complex Living Tissue Constructs

Shabir Hassan, Eduardo Gomez-Reyes, Eduardo Enciso-Martinez, Kun Shi, Jorge Gonzalez Campos, Oscar Yael Perez Soria, Eder Luna-Cerón, Myung Chul Lee, Isaac Garcia-Reyes, Joshua Steakelum, Haziq Jeelani, Luis Enrique García-Rivera, Minsung Cho, Stephanie Sanchez Cortes, Tom Kamperman, Haihang Wang, Jeroen Leijten, Lance Fiondella, Su Ryon Shin*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)
138 Downloads (Pure)

Abstract

Recapitulating inherent heterogeneity and complex microarchitectures within confined print volumes for developing implantable constructs that could maintain their structure in vivo has remained challenging. Here, we present a combinational multimaterial and embedded bioprinting approach to fabricate complex tissue constructs that can be implanted postprinting and retain their three-dimensional (3D) shape in vivo. The microfluidics-based single nozzle printhead with computer-controlled pneumatic pressure valves enables laminar flow-based voxelation of up to seven individual bioinks with rapid switching between various bioinks that can solve alignment issues generated during switching multiple nozzles. To improve the spatial organization of various bioinks, printing fidelity with the z-direction, and printing speed, self-healing and biodegradable colloidal gels as support baths are introduced to build complex geometries. Furthermore, the colloidal gels provide suitable microenvironments like native extracellular matrices (ECMs) for achieving cell growths and fast host cell invasion via interconnected microporous networks in vitro and in vivo. Multicompartment microfibers (i.e., solid, core-shell, or donut shape), composed of two different bioink fractions with various lengths or their intravolume space filled by two, four, and six bioink fractions, are successfully printed in the ECM-like support bath. We also print various acellular complex geometries such as pyramids, spirals, and perfusable branched/linear vessels. Successful fabrication of vascularized liver and skeletal muscle tissue constructs show albumin secretion and bundled muscle mimic fibers, respectively. The interconnected microporous networks of colloidal gels result in maintaining printed complex geometries while enabling rapid cell infiltration, in vivo.

Original languageEnglish
Pages (from-to)51602-51618
Number of pages17
JournalACS Applied Materials and Interfaces
Volume14
Issue number46
DOIs
Publication statusPublished - 8 Nov 2022

Keywords

  • 3D bioprinting
  • colloidal hydrogels
  • compartmentalized bioprinting
  • multimaterial extrusion bioprinting
  • vascular scaffolds
  • 2023 OA procedure

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