Turbulent flow chromatography for the reduction of matrix effects in electrospray ionization mass spectrometry-based enzyme assays

A. Liesener, U. Karst

    Research output: Contribution to journalArticleAcademic

    14 Citations (Scopus)

    Abstract

    Turbulent flow chromatography (TFC) is presented as a means to reduce ion suppression in simultaneous multianalyte mass spectrometric bioassays. In this study, the effects of enzymes present in the sample on the signal response of five analytes were simultaneously investigated over a protein content range from 0 to 38 µg/mL by means of direct flow injection MS. As model enzymes, trypsin, thrombin, and chymotrypsin were selected. Without employment of TFC, both signal suppression and signal enhancement, depending on the nature of the analyte and the amount of matrix in the sample, were observed. Generally, these matrix effects were found to be intolerably large. The deviation from the mean signal response as a measure of deterioration was found to be between 14 and 112%. The addition of an excess of methanol as means of sample clean-up was investigated and found not to be sufficient. By employing TFC for online sample preparation, it was possible to reduce the matrix effects to a minimum for all model systems investigated. In case of trypsin the distortion could be lowered from 41.9 to 2.6%. Thus, TFC is considered to be a highly valuable tool for improving the sensitivity and reliability in the monitoring of enzymatic conversions by means of MS.
    Original languageUndefined
    Pages (from-to)1658-1665
    JournalJournal of separation science
    Volume28
    Issue number14
    DOIs
    Publication statusPublished - 2005

    Keywords

    • Electrospray ionization
    • On-line sample preparation
    • Mass spectrometry
    • Matrix effects
    • IR-72032
    • Turbulent flow chromatography

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