Abstract
In vitro test systems for extracorporeal membrane oxygenation (mock loop) represent an interesting alternative to complex and expensive in vivo test systems to analyze the pathomechanisms leading to insufficient biocompatibility. Data on mock loop systems are limited, and operation times are constricted to a maximum duration of 6 hr. This study aims at a 12 hr operation time and frequent monitoring of markers for insufficient biocompatibility in two experimental settings. Porcine blood circulated in a mock loop without any modifications, or the circuit was operated with a CO 2 -enhanced gas (5% CO 2 /21% O 2 /74% N 2 ) in combination with a nutrient solution (phosphate-adenine-glucose-guanosine-saline-mannitol). Coagulation parameters changed over time without differences between the two groups. In the unmodified test setting, a pH increase was detected after 1 hr, followed by significantly increased levels of free hemoglobin as a marker for hemolysis and elevated numbers of activated platelets, which correlate with detected von Willebrand factor, microparticles, and interleukin-β. Proinflammatory cytokine levels were significantly increased after 12 hr. In contrast, these parameters remained constant in the modified test setting providing proof of a stable operating in vitro mock loop system with an extended/prolonged operation time.
| Original language | English |
|---|---|
| Pages (from-to) | 548-555 |
| Number of pages | 8 |
| Journal | ASAIO Journal |
| Volume | 61 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 Oct 2015 |
| Externally published | Yes |
Fingerprint
Keywords
- 12 hr
- biocompatibility
- extracorporeal membrane oxygenation
- in vitro
Cite this
}
Twelve hours in vitro biocompatibility testing of membrane oxygenators. / Bleilevens, Christian; Grottke, Oliver; Tillmann, Sabine; Honickel, Markus; Kopp, RüEdger; Arens, Jutta; Rossaint, Rolf.
In: ASAIO Journal, Vol. 61, No. 5, 01.10.2015, p. 548-555.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Twelve hours in vitro biocompatibility testing of membrane oxygenators
AU - Bleilevens, Christian
AU - Grottke, Oliver
AU - Tillmann, Sabine
AU - Honickel, Markus
AU - Kopp, RüEdger
AU - Arens, Jutta
AU - Rossaint, Rolf
PY - 2015/10/1
Y1 - 2015/10/1
N2 - In vitro test systems for extracorporeal membrane oxygenation (mock loop) represent an interesting alternative to complex and expensive in vivo test systems to analyze the pathomechanisms leading to insufficient biocompatibility. Data on mock loop systems are limited, and operation times are constricted to a maximum duration of 6 hr. This study aims at a 12 hr operation time and frequent monitoring of markers for insufficient biocompatibility in two experimental settings. Porcine blood circulated in a mock loop without any modifications, or the circuit was operated with a CO 2 -enhanced gas (5% CO 2 /21% O 2 /74% N 2 ) in combination with a nutrient solution (phosphate-adenine-glucose-guanosine-saline-mannitol). Coagulation parameters changed over time without differences between the two groups. In the unmodified test setting, a pH increase was detected after 1 hr, followed by significantly increased levels of free hemoglobin as a marker for hemolysis and elevated numbers of activated platelets, which correlate with detected von Willebrand factor, microparticles, and interleukin-β. Proinflammatory cytokine levels were significantly increased after 12 hr. In contrast, these parameters remained constant in the modified test setting providing proof of a stable operating in vitro mock loop system with an extended/prolonged operation time.
AB - In vitro test systems for extracorporeal membrane oxygenation (mock loop) represent an interesting alternative to complex and expensive in vivo test systems to analyze the pathomechanisms leading to insufficient biocompatibility. Data on mock loop systems are limited, and operation times are constricted to a maximum duration of 6 hr. This study aims at a 12 hr operation time and frequent monitoring of markers for insufficient biocompatibility in two experimental settings. Porcine blood circulated in a mock loop without any modifications, or the circuit was operated with a CO 2 -enhanced gas (5% CO 2 /21% O 2 /74% N 2 ) in combination with a nutrient solution (phosphate-adenine-glucose-guanosine-saline-mannitol). Coagulation parameters changed over time without differences between the two groups. In the unmodified test setting, a pH increase was detected after 1 hr, followed by significantly increased levels of free hemoglobin as a marker for hemolysis and elevated numbers of activated platelets, which correlate with detected von Willebrand factor, microparticles, and interleukin-β. Proinflammatory cytokine levels were significantly increased after 12 hr. In contrast, these parameters remained constant in the modified test setting providing proof of a stable operating in vitro mock loop system with an extended/prolonged operation time.
KW - 12 hr
KW - biocompatibility
KW - extracorporeal membrane oxygenation
KW - in vitro
UR - http://www.scopus.com/inward/record.url?scp=84954130445&partnerID=8YFLogxK
U2 - 10.1097/MAT.0000000000000252
DO - 10.1097/MAT.0000000000000252
M3 - Article
VL - 61
SP - 548
EP - 555
JO - ASAIO Journal
JF - ASAIO Journal
SN - 1058-2916
IS - 5
ER -