Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores

E. Krioukov, D.J.W. Klunder, A. Driessen, Jan Greve, Cornelis Otto

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Abstract

Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.
Original languageUndefined
Article number10.1016/j.talanta.2004.02.048
Pages (from-to)1086-1090
Number of pages5
JournalTalanta
Volume65
Issue number5
DOIs
Publication statusPublished - 15 Mar 2005

Keywords

  • IR-62263
  • Biosensor
  • Microcavity
  • Fluorescence
  • METIS-222623
  • IOMS-SNS: SENSORS
  • EWI-12334
  • Integrated Optics
  • Two photons

Cite this

Krioukov, E. ; Klunder, D.J.W. ; Driessen, A. ; Greve, Jan ; Otto, Cornelis. / Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores. In: Talanta. 2005 ; Vol. 65, No. 5. pp. 1086-1090.
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abstract = "Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.",
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Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores. / Krioukov, E.; Klunder, D.J.W.; Driessen, A.; Greve, Jan; Otto, Cornelis.

In: Talanta, Vol. 65, No. 5, 10.1016/j.talanta.2004.02.048, 15.03.2005, p. 1086-1090.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores

AU - Krioukov, E.

AU - Klunder, D.J.W.

AU - Driessen, A.

AU - Greve, Jan

AU - Otto, Cornelis

PY - 2005/3/15

Y1 - 2005/3/15

N2 - Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.

AB - Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.

KW - IR-62263

KW - Biosensor

KW - Microcavity

KW - Fluorescence

KW - METIS-222623

KW - IOMS-SNS: SENSORS

KW - EWI-12334

KW - Integrated Optics

KW - Two photons

U2 - 10.1016/j.talanta.2004.02.048

DO - 10.1016/j.talanta.2004.02.048

M3 - Article

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M1 - 10.1016/j.talanta.2004.02.048

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