Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores

E. Krioukov, D.J.W. Klunder, A. Driessen, Jan Greve, Cornelis Otto

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Abstract

Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.
Original languageEnglish
Article number10.1016/j.talanta.2004.02.048
Pages (from-to)1086-1090
Number of pages5
JournalTalanta
Volume65
Issue number5
DOIs
Publication statusPublished - 15 Mar 2005

Keywords

  • IR-62263
  • Biosensor
  • Microcavity
  • Fluorescence
  • METIS-222623
  • IOMS-SNS: SENSORS
  • EWI-12334
  • Integrated Optics
  • Two photons

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