Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores

E. Krioukov; D.J.W. Klunder; A. Driessen; Jan Greve; Cornelis Otto

doi:10.1016/j.talanta.2004.02.048

Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores

E. Krioukov
, D.J.W. Klunder
, A. Driessen
, Jan Greve
, Cornelis Otto

Research output: Contribution to journal › Article › Academic › peer-review

11 Citations (Scopus)

5 Downloads (Pure)

Abstract

Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.

Original language	English
Article number	10.1016/j.talanta.2004.02.048
Pages (from-to)	1086-1090
Number of pages	5
Journal	Talanta
Volume	65
Issue number	5
DOIs	https://doi.org/10.1016/j.talanta.2004.02.048
Publication status	Published - 15 Mar 2005

Keywords

IR-62263
Biosensor
Microcavity
Fluorescence
METIS-222623
IOMS-SNS: SENSORS
EWI-12334
Integrated Optics
Two photons

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http://eprints.eemcs.utwente.nl/secure2/12334/01/Krioukov-Klunder-Driessen-Greve-Otto%2520-%2520Two%2520photon%2520%2520Elsevier-Talanta%252065-2005%252C%25201086-1090.pdf

Cite this

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title = "Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores",

abstract = "Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.",

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author = "E. Krioukov and D.J.W. Klunder and A. Driessen and Jan Greve and Cornelis Otto",

year = "2005",

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doi = "10.1016/j.talanta.2004.02.048",

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volume = "65",

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TY - JOUR

T1 - Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores

AU - Krioukov, E.

AU - Klunder, D.J.W.

AU - Driessen, A.

AU - Greve, Jan

AU - Otto, Cornelis

PY - 2005/3/15

Y1 - 2005/3/15

N2 - Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.

AB - Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.

KW - IR-62263

KW - Biosensor

KW - Microcavity

KW - Fluorescence

KW - METIS-222623

KW - IOMS-SNS: SENSORS

KW - EWI-12334

KW - Integrated Optics

KW - Two photons

U2 - 10.1016/j.talanta.2004.02.048

DO - 10.1016/j.talanta.2004.02.048

M3 - Article

SN - 0039-9140

VL - 65

SP - 1086

EP - 1090

JO - Talanta

JF - Talanta

IS - 5

M1 - 10.1016/j.talanta.2004.02.048

ER -

Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores

Abstract

Keywords

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