Ultra-rapid separation of an angiotensin mixture in nanochannels using shear-driven chromatography

Sarah Vankrunkelsven; David Clicq; Deidre Cabooter; Wim de Malsche; Johannes G.E. Gardeniers; Gert Desmet

doi:10.1016/j.chroma.2005.10.017

Ultra-rapid separation of an angiotensin mixture in nanochannels using shear-driven chromatography

Sarah Vankrunkelsven, David Clicq, Deidre Cabooter, Wim de Malsche, Johannes G.E. Gardeniers, Gert Desmet

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The present paper reports on the separation of a mixture of fluorescein isothiocyanate-labeled angiotensin I and II peptides in a shear-driven nanochannel with a C-18-coating and using an eluent consisting of 5% acetonitrile in 0.02 M aqueous phosphate buffer at pH 6.5. The flat-rectangular nanochannel in fused silica consisted of an etched structure in combination with a flat moving wall. The very fast separation kinetics that can be achieved in a nanochannel allowed to separate the angiotensin peptides in less then 0.2 s in a distance of only 1.8 mm. Plate heights as small as 0.4 mu m were calculated after substraction of the injection effect. (c) 2006 Elsevier B.V. All rights reserved.

Original language	Undefined
Article number	10.1016/j.chroma.2005.10.017
Pages (from-to)	96-103
Number of pages	8
Journal	Journal of chromatography A
Volume	1102
Issue number	10/1-2
DOIs	https://doi.org/10.1016/j.chroma.2005.10.017
Publication status	Published - 13 Jan 2006

Keywords

EWI-8367
IR-63736
METIS-237679
BIOS-Micro/nanofluidics

Access to Document

10.1016/j.chroma.2005.10.017

http://eprints.eemcs.utwente.nl/secure2/8367/01/ultra-rapid_separation_of_an_angiotensin....pdf

Cite this

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title = "Ultra-rapid separation of an angiotensin mixture in nanochannels using shear-driven chromatography",

abstract = "The present paper reports on the separation of a mixture of fluorescein isothiocyanate-labeled angiotensin I and II peptides in a shear-driven nanochannel with a C-18-coating and using an eluent consisting of 5% acetonitrile in 0.02 M aqueous phosphate buffer at pH 6.5. The flat-rectangular nanochannel in fused silica consisted of an etched structure in combination with a flat moving wall. The very fast separation kinetics that can be achieved in a nanochannel allowed to separate the angiotensin peptides in less then 0.2 s in a distance of only 1.8 mm. Plate heights as small as 0.4 mu m were calculated after substraction of the injection effect. (c) 2006 Elsevier B.V. All rights reserved.",

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author = "Sarah Vankrunkelsven and David Clicq and Deidre Cabooter and {de Malsche}, Wim and Gardeniers, {Johannes G.E.} and Gert Desmet",

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T1 - Ultra-rapid separation of an angiotensin mixture in nanochannels using shear-driven chromatography

AU - Vankrunkelsven, Sarah

AU - Clicq, David

AU - Cabooter, Deidre

AU - de Malsche, Wim

AU - Gardeniers, Johannes G.E.

AU - Desmet, Gert

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PY - 2006/1/13

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AB - The present paper reports on the separation of a mixture of fluorescein isothiocyanate-labeled angiotensin I and II peptides in a shear-driven nanochannel with a C-18-coating and using an eluent consisting of 5% acetonitrile in 0.02 M aqueous phosphate buffer at pH 6.5. The flat-rectangular nanochannel in fused silica consisted of an etched structure in combination with a flat moving wall. The very fast separation kinetics that can be achieved in a nanochannel allowed to separate the angiotensin peptides in less then 0.2 s in a distance of only 1.8 mm. Plate heights as small as 0.4 mu m were calculated after substraction of the injection effect. (c) 2006 Elsevier B.V. All rights reserved.

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KW - IR-63736

KW - METIS-237679

KW - BIOS-Micro/nanofluidics

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