Ultra-rapid separation of an angiotensin mixture in nanochannels using shear-driven chromatography

Sarah Vankrunkelsven, David Clicq, Deidre Cabooter, Wim de Malsche, Johannes G.E. Gardeniers, Gert Desmet

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36 Citations (Scopus)


The present paper reports on the separation of a mixture of fluorescein isothiocyanate-labeled angiotensin I and II peptides in a shear-driven nanochannel with a C-18-coating and using an eluent consisting of 5% acetonitrile in 0.02 M aqueous phosphate buffer at pH 6.5. The flat-rectangular nanochannel in fused silica consisted of an etched structure in combination with a flat moving wall. The very fast separation kinetics that can be achieved in a nanochannel allowed to separate the angiotensin peptides in less then 0.2 s in a distance of only 1.8 mm. Plate heights as small as 0.4 mu m were calculated after substraction of the injection effect. (c) 2006 Elsevier B.V. All rights reserved.
Original languageUndefined
Article number10.1016/j.chroma.2005.10.017
Pages (from-to)96-103
Number of pages8
JournalJournal of chromatography A
Issue number10/1-2
Publication statusPublished - 13 Jan 2006


  • EWI-8367
  • IR-63736
  • METIS-237679
  • BIOS-Micro/nanofluidics

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