Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion

Lyubov Amitonova, I.V. Fedotov, A.M. Zheltikov

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Stimulated emission depletion (STED) is the key optical technology enabling super-resolution microscopy below the diffraction limit. Here, we demonstrate that modulation of STED in the time domain, combined with properly designed lock-in detection, can radically enhance the contrast of fluorescent images of strongly autofluorescent biotissues. In our experiments, the temporally modulated STED technique, implemented with low-intensity continuous-wave laser sources, is shown to provide an efficient all-optical suppression of a broadband fluorescent background, allowing the contrast of fluorescent images of mammal brain tissues tagged with nitrogen-vacancy diamond to be increased by five orders of magnitude.
Original languageEnglish
Pages (from-to)725-728
JournalOptics letters
Volume40
Issue number5
DOIs
Publication statusPublished - 7 Jan 2015

Fingerprint

stimulated emission
depletion
mammals
continuous wave lasers
brain
diamonds
retarding
broadband
microscopy
modulation
nitrogen
diffraction

Keywords

  • IR-95489
  • METIS-310210

Cite this

Amitonova, Lyubov ; Fedotov, I.V. ; Zheltikov, A.M. / Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion. In: Optics letters. 2015 ; Vol. 40, No. 5. pp. 725-728.
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Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion. / Amitonova, Lyubov; Fedotov, I.V.; Zheltikov, A.M.

In: Optics letters, Vol. 40, No. 5, 07.01.2015, p. 725-728.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Fedotov, I.V.

AU - Zheltikov, A.M.

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AB - Stimulated emission depletion (STED) is the key optical technology enabling super-resolution microscopy below the diffraction limit. Here, we demonstrate that modulation of STED in the time domain, combined with properly designed lock-in detection, can radically enhance the contrast of fluorescent images of strongly autofluorescent biotissues. In our experiments, the temporally modulated STED technique, implemented with low-intensity continuous-wave laser sources, is shown to provide an efficient all-optical suppression of a broadband fluorescent background, allowing the contrast of fluorescent images of mammal brain tissues tagged with nitrogen-vacancy diamond to be increased by five orders of magnitude.

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KW - METIS-310210

U2 - 10.1364/OL.40.000725

DO - 10.1364/OL.40.000725

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