Ultrasensitive Detection and in Situ Imaging of Analytes on Graphene Oxide Analogues Using Enhanced Raman Spectroscopy

Sachin Nair; Jun Gao; Cees Otto; Michael H.G. Duits; Frieder Mugele

doi:10.1021/acs.analchem.1c02581

Ultrasensitive Detection and in Situ Imaging of Analytes on Graphene Oxide Analogues Using Enhanced Raman Spectroscopy

Sachin Nair^*
, Jun Gao
, Cees Otto
, Michael H.G. Duits
, Frieder Mugele^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

262 Downloads (Pure)

Abstract

We demonstrate how algorithm-improved confocal Raman microscopy (ai-CRM), in combination with chemical enhancement by two-dimensional substrates, can be used as an ultrasensitive detection method for rhodamine (R6G) molecules adsorbed from aqueous solutions. After developing a protocol for laser-induced reduction of graphene oxide, followed by noninvasive Raman imaging, a limit of detection (LOD) of 5 × 10^-10M R6G was achieved using ai-CRM. An equivalent subnanomolar LOD was also achieved on another graphene oxide analogue −UV/ozone-oxidized graphene. These record-breaking detection capabilities also enabled us to study the adsorption kinetics and image the spatial distribution of the adsorbed R6G. These findings indicate a strong potential for algorithm-improved graphene-enhanced Raman spectroscopy as a facile method for detecting, imaging, and quantifying trace amounts of adsorbing molecules on a variety of 2D substrates.

Original language	English
Pages (from-to)	12966-12972
Number of pages	7
Journal	Analytical chemistry
Volume	93
Issue number	38
DOIs	https://doi.org/10.1021/acs.analchem.1c02581
Publication status	Published - 28 Sept 2021

Keywords

UT-Hybrid-D

Access to Document

10.1021/acs.analchem.1c02581Licence: CC BY

acs.analchem.1c02581Final published version, 3.27 MBLicence: CC BY

Cite this

@article{8daad08dc9554207aa37d61ed24ad5b0,

title = "Ultrasensitive Detection and in Situ Imaging of Analytes on Graphene Oxide Analogues Using Enhanced Raman Spectroscopy",

abstract = "We demonstrate how algorithm-improved confocal Raman microscopy (ai-CRM), in combination with chemical enhancement by two-dimensional substrates, can be used as an ultrasensitive detection method for rhodamine (R6G) molecules adsorbed from aqueous solutions. After developing a protocol for laser-induced reduction of graphene oxide, followed by noninvasive Raman imaging, a limit of detection (LOD) of 5 × 10-10M R6G was achieved using ai-CRM. An equivalent subnanomolar LOD was also achieved on another graphene oxide analogue −UV/ozone-oxidized graphene. These record-breaking detection capabilities also enabled us to study the adsorption kinetics and image the spatial distribution of the adsorbed R6G. These findings indicate a strong potential for algorithm-improved graphene-enhanced Raman spectroscopy as a facile method for detecting, imaging, and quantifying trace amounts of adsorbing molecules on a variety of 2D substrates.",

keywords = "UT-Hybrid-D",

author = "Sachin Nair and Jun Gao and Cees Otto and Duits, \{Michael H.G.\} and Frieder Mugele",

note = "Funding Information: This work is part of the research program Rock-on-a-Chip with project number i40, which is partly financed by The Netherlands Organization for Scientific Research (NWO) and the Exploratory Research (ExploRe) program of BP plc. We thank B. Ilhan and S. Kumar for carrying out the in situ AFM imaging of (r)GO flakes. Publisher Copyright: {\textcopyright} 2021 The Authors. Published by American Chemical Society",

year = "2021",

month = sep,

day = "28",

doi = "10.1021/acs.analchem.1c02581",

language = "English",

volume = "93",

pages = "12966--12972",

journal = "Analytical chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "38",

}

TY - JOUR

T1 - Ultrasensitive Detection and in Situ Imaging of Analytes on Graphene Oxide Analogues Using Enhanced Raman Spectroscopy

AU - Nair, Sachin

AU - Gao, Jun

AU - Otto, Cees

AU - Duits, Michael H.G.

AU - Mugele, Frieder

N1 - Funding Information: This work is part of the research program Rock-on-a-Chip with project number i40, which is partly financed by The Netherlands Organization for Scientific Research (NWO) and the Exploratory Research (ExploRe) program of BP plc. We thank B. Ilhan and S. Kumar for carrying out the in situ AFM imaging of (r)GO flakes. Publisher Copyright: © 2021 The Authors. Published by American Chemical Society

PY - 2021/9/28

Y1 - 2021/9/28

N2 - We demonstrate how algorithm-improved confocal Raman microscopy (ai-CRM), in combination with chemical enhancement by two-dimensional substrates, can be used as an ultrasensitive detection method for rhodamine (R6G) molecules adsorbed from aqueous solutions. After developing a protocol for laser-induced reduction of graphene oxide, followed by noninvasive Raman imaging, a limit of detection (LOD) of 5 × 10-10M R6G was achieved using ai-CRM. An equivalent subnanomolar LOD was also achieved on another graphene oxide analogue −UV/ozone-oxidized graphene. These record-breaking detection capabilities also enabled us to study the adsorption kinetics and image the spatial distribution of the adsorbed R6G. These findings indicate a strong potential for algorithm-improved graphene-enhanced Raman spectroscopy as a facile method for detecting, imaging, and quantifying trace amounts of adsorbing molecules on a variety of 2D substrates.

AB - We demonstrate how algorithm-improved confocal Raman microscopy (ai-CRM), in combination with chemical enhancement by two-dimensional substrates, can be used as an ultrasensitive detection method for rhodamine (R6G) molecules adsorbed from aqueous solutions. After developing a protocol for laser-induced reduction of graphene oxide, followed by noninvasive Raman imaging, a limit of detection (LOD) of 5 × 10-10M R6G was achieved using ai-CRM. An equivalent subnanomolar LOD was also achieved on another graphene oxide analogue −UV/ozone-oxidized graphene. These record-breaking detection capabilities also enabled us to study the adsorption kinetics and image the spatial distribution of the adsorbed R6G. These findings indicate a strong potential for algorithm-improved graphene-enhanced Raman spectroscopy as a facile method for detecting, imaging, and quantifying trace amounts of adsorbing molecules on a variety of 2D substrates.

KW - UT-Hybrid-D

UR - https://www.scopus.com/pages/publications/85115995540

U2 - 10.1021/acs.analchem.1c02581

DO - 10.1021/acs.analchem.1c02581

M3 - Article

AN - SCOPUS:85115995540

SN - 0003-2700

VL - 93

SP - 12966

EP - 12972

JO - Analytical chemistry

JF - Analytical chemistry

IS - 38

ER -

Ultrasensitive Detection and in Situ Imaging of Analytes on Graphene Oxide Analogues Using Enhanced Raman Spectroscopy

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this