Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells

Katarina Radosevic, Bart G. de Grooth, Jan Greve

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    The studies described in this manuscript have been aimed to obtain a better insight into the cytotoxic interaction between human Natural Killer (NK) cells and K562 target cells1. In order to obtain information about crucial processes leading to target cell death, the processes taking place in the target cell during a killer cell attack were studied using different optical techniques. Furthermore, flow cytometric methods for determination of the phenotype and the cytotoxic activity of killer cells were developed.
    Original languageEnglish
    Title of host publicationFlow and Image Cytometry
    EditorsAlain Jacquamin-Sablon
    Place of PublicationBerlin, Heidelberg
    PublisherSpringer
    Pages53-68
    Number of pages16
    ISBN (Electronic)978-3-642-61115-5
    ISBN (Print)978-3-642-64701-7
    DOIs
    Publication statusPublished - 1996
    EventNATO Advanced Study Institute on Progress in Flow and Image Cytometry 1995 - Villeneuf, France
    Duration: 15 May 199519 May 1995

    Publication series

    NameNATO Advanced Science Institutes Series H: Cell Biology
    PublisherSpringer
    Volume95
    ISSN (Print)1010-8793

    Conference

    ConferenceNATO Advanced Study Institute on Progress in Flow and Image Cytometry 1995
    CountryFrance
    CityVilleneuf
    Period15/05/9519/05/95

    Keywords

    • METIS-129725
    • METIS-129959
    • K562 Cell
    • Human Natural Killer Cell
    • Flow Cytometric Method
    • Rhodamine Phalloidin
    • Light Scattering Signal

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  • Cite this

    Radosevic, K., de Grooth, B. G., & Greve, J. (1996). Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells. In A. Jacquamin-Sablon (Ed.), Flow and Image Cytometry (pp. 53-68). (NATO Advanced Science Institutes Series H: Cell Biology ; Vol. 95). Berlin, Heidelberg: Springer. https://doi.org/10.1007/978-3-642-61115-5_4