Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells

Katarina Radosevic; Bart G. de Grooth; Jan Greve

doi:10.1007/978-3-642-61115-5_4

Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells

Katarina Radosevic, Bart G. de Grooth, Jan Greve

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

The studies described in this manuscript have been aimed to obtain a better insight into the cytotoxic interaction between human Natural Killer (NK) cells and K562 target cells1. In order to obtain information about crucial processes leading to target cell death, the processes taking place in the target cell during a killer cell attack were studied using different optical techniques. Furthermore, flow cytometric methods for determination of the phenotype and the cytotoxic activity of killer cells were developed.

Original language	English
Title of host publication	Flow and Image Cytometry
Editors	Alain Jacquamin-Sablon
Place of Publication	Berlin, Heidelberg
Publisher	Springer
Pages	53-68
Number of pages	16
ISBN (Electronic)	978-3-642-61115-5
ISBN (Print)	978-3-642-64701-7
DOIs	https://doi.org/10.1007/978-3-642-61115-5_4
Publication status	Published - 1996
Event	NATO Advanced Study Institute on Progress in Flow and Image Cytometry 1995 - Villeneuf, France Duration: 15 May 1995 → 19 May 1995

Publication series

Name	NATO Advanced Science Institutes Series H: Cell Biology
Publisher	Springer
Volume	95
ISSN (Print)	1010-8793

Conference

Conference	NATO Advanced Study Institute on Progress in Flow and Image Cytometry 1995
Country	France
City	Villeneuf
Period	15/05/95 → 19/05/95

Keywords

METIS-129725
METIS-129959
K562 Cell
Human Natural Killer Cell
Flow Cytometric Method
Rhodamine Phalloidin
Light Scattering Signal

Access to Document

10.1007/978-3-642-61115-5_4

Cite this

Radosevic, K., de Grooth, B. G., & Greve, J. (1996). Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells. In A. Jacquamin-Sablon (Ed.), Flow and Image Cytometry (pp. 53-68). (NATO Advanced Science Institutes Series H: Cell Biology ; Vol. 95). Springer. https://doi.org/10.1007/978-3-642-61115-5_4

@inproceedings{69665253cf164e8ebba9efb2a645a7af,

title = "Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells",

abstract = "The studies described in this manuscript have been aimed to obtain a better insight into the cytotoxic interaction between human Natural Killer (NK) cells and K562 target cells1. In order to obtain information about crucial processes leading to target cell death, the processes taking place in the target cell during a killer cell attack were studied using different optical techniques. Furthermore, flow cytometric methods for determination of the phenotype and the cytotoxic activity of killer cells were developed.",

keywords = "METIS-129725, METIS-129959, K562 Cell, Human Natural Killer Cell, Flow Cytometric Method, Rhodamine Phalloidin, Light Scattering Signal",

author = "Katarina Radosevic and {de Grooth}, {Bart G.} and Jan Greve",

year = "1996",

doi = "10.1007/978-3-642-61115-5_4",

language = "English",

isbn = "978-3-642-64701-7",

series = "NATO Advanced Science Institutes Series H: Cell Biology ",

publisher = "Springer",

pages = "53--68",

editor = "Alain Jacquamin-Sablon",

booktitle = "Flow and Image Cytometry",

address = "Netherlands",

note = "NATO Advanced Study Institute on Progress in Flow and Image Cytometry 1995 ; Conference date: 15-05-1995 Through 19-05-1995",

}

Radosevic, K, de Grooth, BG & Greve, J 1996, Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells. in A Jacquamin-Sablon (ed.), Flow and Image Cytometry. NATO Advanced Science Institutes Series H: Cell Biology , vol. 95, Springer, Berlin, Heidelberg, pp. 53-68, NATO Advanced Study Institute on Progress in Flow and Image Cytometry 1995, Villeneuf, France, 15/05/95. https://doi.org/10.1007/978-3-642-61115-5_4

Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells. / Radosevic, Katarina; de Grooth, Bart G.; Greve, Jan.

Flow and Image Cytometry. ed. / Alain Jacquamin-Sablon. Berlin, Heidelberg : Springer, 1996. p. 53-68 (NATO Advanced Science Institutes Series H: Cell Biology ; Vol. 95).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells

AU - Radosevic, Katarina

AU - de Grooth, Bart G.

AU - Greve, Jan

PY - 1996

Y1 - 1996

N2 - The studies described in this manuscript have been aimed to obtain a better insight into the cytotoxic interaction between human Natural Killer (NK) cells and K562 target cells1. In order to obtain information about crucial processes leading to target cell death, the processes taking place in the target cell during a killer cell attack were studied using different optical techniques. Furthermore, flow cytometric methods for determination of the phenotype and the cytotoxic activity of killer cells were developed.

AB - The studies described in this manuscript have been aimed to obtain a better insight into the cytotoxic interaction between human Natural Killer (NK) cells and K562 target cells1. In order to obtain information about crucial processes leading to target cell death, the processes taking place in the target cell during a killer cell attack were studied using different optical techniques. Furthermore, flow cytometric methods for determination of the phenotype and the cytotoxic activity of killer cells were developed.

KW - METIS-129725

KW - METIS-129959

KW - K562 Cell

KW - Human Natural Killer Cell

KW - Flow Cytometric Method

KW - Rhodamine Phalloidin

KW - Light Scattering Signal

U2 - 10.1007/978-3-642-61115-5_4

DO - 10.1007/978-3-642-61115-5_4

M3 - Conference contribution

SN - 978-3-642-64701-7

T3 - NATO Advanced Science Institutes Series H: Cell Biology

SP - 53

EP - 68

BT - Flow and Image Cytometry

A2 - Jacquamin-Sablon, Alain

PB - Springer

CY - Berlin, Heidelberg

T2 - NATO Advanced Study Institute on Progress in Flow and Image Cytometry 1995

Y2 - 15 May 1995 through 19 May 1995

ER -

Radosevic K, de Grooth BG, Greve J. Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells. In Jacquamin-Sablon A, editor, Flow and Image Cytometry. Berlin, Heidelberg: Springer. 1996. p. 53-68. (NATO Advanced Science Institutes Series H: Cell Biology ). https://doi.org/10.1007/978-3-642-61115-5_4

Use of different cytometric techniques to study the cytotoxic interaction between human natural killer cells and K562 target cells

Abstract

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Keywords

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