Vascularized Tissue Blocks Using a Suspension 3D Printed Spheroid Blood Vessel

V.D. Trikalitis, F. Stein, J. Perea Paizal, N. Salehi Nik, J. Rouwkema

    Research output: Contribution to conferenceAbstract

    Abstract

    In order for engineered tissue grafts and eventually organs to successfully integrate in a clinical setting, a functional vascular network is imperative. Without vasculature, the tissue constructs cannot receive nutrients essential for their survival, but also lack the stimuli that determine the tissue’s biophysical properties i.e. cell fate determination, cell to cell junctions, and cell orientation. In order for the vascular network to functionally connect to the patient, a hierarchical organization, resembling the vascular tree, is important. From previous studies it is known that fluid flow is a crucial component in controlling the formation of the vascular tree, and that the organization of the vascular network can be further controlled using gradients of angiogenic growth factors such as VEGF.
    By utilizing spheroid bioprinting within a microgel suspension, an artificial vessel structure was assembled. The deposited spheroids maintained viability and fused over time into perfusable vessels.The subsequent formation of small-diameter vascular structures and capillaries was regulated by an on-demand flow through the bioprinted vessel, resulting in controllable fluid flow shear stresses. Furthermore, VEGF was spatially patterned in the tissue block by locally doping the suspension with growth factor releasing microparticles. By varying both these stimuli, the location of vascular sprout formation and subsequent growth of the new vascular structures could be influenced.
    This spheroid 3D bioprinting platform offers a dynamic, customizable and accurate method to trigger and control the process of angiogenesis in vitro. By stimulating an artificial blood vessel with controlled fluid flow and growth factor gradients, a vascular complex vascular network can be produced and modulated. The combination of this approach with a gradual replacement of the microgel suspension with cells, can pave the way for the production of vascularized tissue blocks.
    Original languageEnglish
    Publication statusPublished - 24 Jan 2018
    Event5th TERMIS World Congress 2018: Integration of Industry, Government, and Academia for Regenerative Medicine - Kyoto International Conference Center , Kyoto, Japan
    Duration: 4 Sep 20187 Sep 2018
    Conference number: 5
    https://www.termis.org/wc2018/

    Conference

    Conference5th TERMIS World Congress 2018
    Abbreviated titleTERMIS-WC
    CountryJapan
    CityKyoto
    Period4/09/187/09/18
    Internet address

    Fingerprint

    Blood Vessels
    Suspensions
    Bioprinting
    Intercellular Signaling Peptides and Proteins
    Vascular Endothelial Growth Factor A
    Blood Substitutes
    Intercellular Junctions
    Angiogenesis Inducing Agents
    Transplants
    Food
    Survival
    Growth

    Keywords

    • TISSUE
    • VASCULARIZATION
    • 3D PRINTING
    • SPHEROIDS
    • EMBEDDED
    • LIQUID LIKE HYDROGEL
    • Suspension

    Cite this

    Trikalitis, V. D., Stein, F., Perea Paizal, J., Salehi Nik, N., & Rouwkema, J. (2018). Vascularized Tissue Blocks Using a Suspension 3D Printed Spheroid Blood Vessel. Abstract from 5th TERMIS World Congress 2018, Kyoto, Japan.
    Trikalitis, V.D. ; Stein, F. ; Perea Paizal, J. ; Salehi Nik, N. ; Rouwkema, J. / Vascularized Tissue Blocks Using a Suspension 3D Printed Spheroid Blood Vessel. Abstract from 5th TERMIS World Congress 2018, Kyoto, Japan.
    @conference{adbe57eb0a804d33b995919137467478,
    title = "Vascularized Tissue Blocks Using a Suspension 3D Printed Spheroid Blood Vessel",
    abstract = "In order for engineered tissue grafts and eventually organs to successfully integrate in a clinical setting, a functional vascular network is imperative. Without vasculature, the tissue constructs cannot receive nutrients essential for their survival, but also lack the stimuli that determine the tissue’s biophysical properties i.e. cell fate determination, cell to cell junctions, and cell orientation. In order for the vascular network to functionally connect to the patient, a hierarchical organization, resembling the vascular tree, is important. From previous studies it is known that fluid flow is a crucial component in controlling the formation of the vascular tree, and that the organization of the vascular network can be further controlled using gradients of angiogenic growth factors such as VEGF. By utilizing spheroid bioprinting within a microgel suspension, an artificial vessel structure was assembled. The deposited spheroids maintained viability and fused over time into perfusable vessels.The subsequent formation of small-diameter vascular structures and capillaries was regulated by an on-demand flow through the bioprinted vessel, resulting in controllable fluid flow shear stresses. Furthermore, VEGF was spatially patterned in the tissue block by locally doping the suspension with growth factor releasing microparticles. By varying both these stimuli, the location of vascular sprout formation and subsequent growth of the new vascular structures could be influenced. This spheroid 3D bioprinting platform offers a dynamic, customizable and accurate method to trigger and control the process of angiogenesis in vitro. By stimulating an artificial blood vessel with controlled fluid flow and growth factor gradients, a vascular complex vascular network can be produced and modulated. The combination of this approach with a gradual replacement of the microgel suspension with cells, can pave the way for the production of vascularized tissue blocks.",
    keywords = "TISSUE, VASCULARIZATION, 3D PRINTING, SPHEROIDS, EMBEDDED, LIQUID LIKE HYDROGEL, Suspension",
    author = "V.D. Trikalitis and F. Stein and {Perea Paizal}, J. and {Salehi Nik}, N. and J. Rouwkema",
    year = "2018",
    month = "1",
    day = "24",
    language = "English",
    note = "5th TERMIS World Congress 2018 : Integration of Industry, Government, and Academia for Regenerative Medicine, TERMIS-WC ; Conference date: 04-09-2018 Through 07-09-2018",
    url = "https://www.termis.org/wc2018/",

    }

    Trikalitis, VD, Stein, F, Perea Paizal, J, Salehi Nik, N & Rouwkema, J 2018, 'Vascularized Tissue Blocks Using a Suspension 3D Printed Spheroid Blood Vessel' 5th TERMIS World Congress 2018, Kyoto, Japan, 4/09/18 - 7/09/18, .

    Vascularized Tissue Blocks Using a Suspension 3D Printed Spheroid Blood Vessel. / Trikalitis, V.D.; Stein, F.; Perea Paizal, J.; Salehi Nik, N.; Rouwkema, J.

    2018. Abstract from 5th TERMIS World Congress 2018, Kyoto, Japan.

    Research output: Contribution to conferenceAbstract

    TY - CONF

    T1 - Vascularized Tissue Blocks Using a Suspension 3D Printed Spheroid Blood Vessel

    AU - Trikalitis, V.D.

    AU - Stein, F.

    AU - Perea Paizal, J.

    AU - Salehi Nik, N.

    AU - Rouwkema, J.

    PY - 2018/1/24

    Y1 - 2018/1/24

    N2 - In order for engineered tissue grafts and eventually organs to successfully integrate in a clinical setting, a functional vascular network is imperative. Without vasculature, the tissue constructs cannot receive nutrients essential for their survival, but also lack the stimuli that determine the tissue’s biophysical properties i.e. cell fate determination, cell to cell junctions, and cell orientation. In order for the vascular network to functionally connect to the patient, a hierarchical organization, resembling the vascular tree, is important. From previous studies it is known that fluid flow is a crucial component in controlling the formation of the vascular tree, and that the organization of the vascular network can be further controlled using gradients of angiogenic growth factors such as VEGF. By utilizing spheroid bioprinting within a microgel suspension, an artificial vessel structure was assembled. The deposited spheroids maintained viability and fused over time into perfusable vessels.The subsequent formation of small-diameter vascular structures and capillaries was regulated by an on-demand flow through the bioprinted vessel, resulting in controllable fluid flow shear stresses. Furthermore, VEGF was spatially patterned in the tissue block by locally doping the suspension with growth factor releasing microparticles. By varying both these stimuli, the location of vascular sprout formation and subsequent growth of the new vascular structures could be influenced. This spheroid 3D bioprinting platform offers a dynamic, customizable and accurate method to trigger and control the process of angiogenesis in vitro. By stimulating an artificial blood vessel with controlled fluid flow and growth factor gradients, a vascular complex vascular network can be produced and modulated. The combination of this approach with a gradual replacement of the microgel suspension with cells, can pave the way for the production of vascularized tissue blocks.

    AB - In order for engineered tissue grafts and eventually organs to successfully integrate in a clinical setting, a functional vascular network is imperative. Without vasculature, the tissue constructs cannot receive nutrients essential for their survival, but also lack the stimuli that determine the tissue’s biophysical properties i.e. cell fate determination, cell to cell junctions, and cell orientation. In order for the vascular network to functionally connect to the patient, a hierarchical organization, resembling the vascular tree, is important. From previous studies it is known that fluid flow is a crucial component in controlling the formation of the vascular tree, and that the organization of the vascular network can be further controlled using gradients of angiogenic growth factors such as VEGF. By utilizing spheroid bioprinting within a microgel suspension, an artificial vessel structure was assembled. The deposited spheroids maintained viability and fused over time into perfusable vessels.The subsequent formation of small-diameter vascular structures and capillaries was regulated by an on-demand flow through the bioprinted vessel, resulting in controllable fluid flow shear stresses. Furthermore, VEGF was spatially patterned in the tissue block by locally doping the suspension with growth factor releasing microparticles. By varying both these stimuli, the location of vascular sprout formation and subsequent growth of the new vascular structures could be influenced. This spheroid 3D bioprinting platform offers a dynamic, customizable and accurate method to trigger and control the process of angiogenesis in vitro. By stimulating an artificial blood vessel with controlled fluid flow and growth factor gradients, a vascular complex vascular network can be produced and modulated. The combination of this approach with a gradual replacement of the microgel suspension with cells, can pave the way for the production of vascularized tissue blocks.

    KW - TISSUE

    KW - VASCULARIZATION

    KW - 3D PRINTING

    KW - SPHEROIDS

    KW - EMBEDDED

    KW - LIQUID LIKE HYDROGEL

    KW - Suspension

    M3 - Abstract

    ER -

    Trikalitis VD, Stein F, Perea Paizal J, Salehi Nik N, Rouwkema J. Vascularized Tissue Blocks Using a Suspension 3D Printed Spheroid Blood Vessel. 2018. Abstract from 5th TERMIS World Congress 2018, Kyoto, Japan.