Vesicle-based artificial cells: materials, construction methods and applications

Yao Lu, Giulia Allegri, Jurriaan Huskens*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

40 Citations (Scopus)
34 Downloads (Pure)


The construction of artificial cells with specific cell-mimicking functions helps to explore complex biological processes and cell functions in natural cell systems and provides an insight into the origins of life. Bottom-up methods are widely used for engineering artificial cells based on vesicles by the in vitro assembly of biomimetic materials. In this review, the design of artificial cells with a specific function is discussed, by considering the selection of synthetic materials and construction technologies. First, a range of biomimetic materials for artificial cells is reviewed, including lipid, polymeric and hybrid lipid/copolymer materials. Biomaterials extracted from natural cells are also covered in this part. Then, the formation of microscale, giant unilamellar vesicles (GUVs) is reviewed based on different technologies, including gentle hydration, electro-formation, phase transfer and microfluidic methods. Subsequently, applications of artificial cells based on single vesicles or vesicle networks are addressed for mimicking cell behaviors and signaling processes. Microreactors for synthetic biology and cell–cell communication are highlighted here as well. Finally, current challenges and future trends for the development and applications of artificial cells are described.
Original languageEnglish
Pages (from-to)892-907
Number of pages16
JournalMaterials horizons
Issue number3
Early online date26 Nov 2021
Publication statusPublished - 1 Mar 2022


  • UT-Hybrid-D


Dive into the research topics of 'Vesicle-based artificial cells: materials, construction methods and applications'. Together they form a unique fingerprint.

Cite this