Vibrational phase contrast CARS microscopy for quantitative analysis

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Abstract

In biological samples the resonant CARS signal of less abundant constituents can be overwhelmed by the nonresonant background, preventing detection of those molecules. We demonstrate a method to obtain the phase of the oscillators in the focal volume that allows discrimination of those hidden molecules. The phase is measured with respect to the local excitation fields using a cascaded phase-preserving chain. It is measured point-bypoint and takes into account refractive index changes in the sample, phase curvature over the field-of-view and interferometric instabilities. The detection of the phase of the vibrational motion can be regarded as a vibrational extension of the linear (refractive index) phase contrast microscopy introduced by Zernike around 1933
Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences X
EditorsAmmasi Periasamy, Peter T.C. So, Karsten Konig
Place of PublicationBellingham, WA
PublisherSPIE
Number of pages9
ISBN (Print)9780819479655
DOIs
Publication statusPublished - 2010
EventSPIE Photonics West 2010: Connecting minds for global solutions - The Moscone Center, San Francisco, United States
Duration: 23 Jan 201028 Jan 2010

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume7569
ISSN (Print)0277-786X

Conference

ConferenceSPIE Photonics West 2010
CountryUnited States
CitySan Francisco
Period23/01/1028/01/10

Keywords

  • METIS-267055
  • IR-77699

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    Jurna, M., Garbacik, E. T., Korterik, J. P., Otto, C., Herek, J. L., & Offerhaus, H. L. (2010). Vibrational phase contrast CARS microscopy for quantitative analysis. In A. Periasamy, P. T. C. So, & K. Konig (Eds.), Multiphoton Microscopy in the Biomedical Sciences X [75690F] (Proceedings of SPIE; Vol. 7569). Bellingham, WA: SPIE. https://doi.org/10.1117/12.840178