Virus-based organelles: enzyme and DNA-based virus nanostructures and their cellular interactions

Mark Vincent de Ruiter

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

Abstract

Various protein-based organelles exist in nature that are involved in a wide variety of different metabolic pathways. However, the exact benefit and function of protein-based organelles is still not completely understood. In the first part of this thesis, we create virus-based nanoreactors to mimic these organelles and to gain more understanding about the benefits of performing reactions inside a protein capsid. These nanoreactors are made by encapsulating various different enzymes in the protein capsid of the cowpea chlorotic mottle virus (CCMV). Furthermore, we investigated their potential use for medical applications by studying their extracellular and intracellular interaction with cancer cells.
To improve on the current design, it is important to understand both the assembly of the virus(-like) particles and their cellular interaction. Therefore, in the second part of this thesis we aim to understand the assembly processes of a virus protein capsid and to find the optimal shape for virus-based nanostructures for cellular uptake. Here the assembly of the capsid proteins of CCMV around various lengths and forms of DNA is studied, which resulted in the formation of various virus-based nanostructures with different (new) geometries. These structures are used to study the shape depended cellular uptake and fate in in different cell lines.
The results presented in this thesis form a solid basis for further research in the use of CCMV for medical and other applications. The found benefits of the virus-based nanoreactors and nanostructures will hopefully lead to the development of new drug formulations. Thus viruses will not only make us ill, but eventually make us better instead.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Cornelissen, Jeroen, Supervisor
Award date1 Feb 2019
Place of PublicationEnschede
Publisher
Print ISBNs978-90-365-4703-1
DOIs
Publication statusPublished - 1 Feb 2019

Fingerprint

DNA Viruses
Nanostructures
Organelles
Bromovirus
Capsid Proteins
Viruses
Enzymes
Drug Compounding
Metabolic Networks and Pathways
Virion
Proteins
Cell Line
DNA
Research
Neoplasms

Cite this

de Ruiter, Mark Vincent. / Virus-based organelles : enzyme and DNA-based virus nanostructures and their cellular interactions. Enschede : University of Twente, 2019. 187 p.
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Virus-based organelles : enzyme and DNA-based virus nanostructures and their cellular interactions. / de Ruiter, Mark Vincent.

Enschede : University of Twente, 2019. 187 p.

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

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