Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging

D.V. Dorokhin, S.H. Hsu, N. Tomczak, Christian Blum, Vinod Subramaniam, Jurriaan Huskens, David Reinhoudt, Aldrik Velders, Gyula J. Vancso

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Abstract

Detection of an analyte via supramolecular host–guest binding and quantum dot (QD)-based fluorescence resonance energy transfer (FRET) signal transduction mechanism is demonstrated. Surface patterns consisting of CdSe/ZnS QDs functionalized at their periphery with β-cyclodextrin (β-CD) were obtained by immobilization of the QDs from solution onto glass substrates patterned with adamantyl-terminated poly(propylene imine) dendrimeric “glue.” Subsequent formation of host–guest complexes between vacant β-CD on the QD surface and an adamantyl-functionalized lissamine rhodamine resulting in FRET was confirmed by fluorescence microscopy, spectroscopy, and fluorescence lifetime imaging microscopy (FLIM).
Original languageEnglish
Pages (from-to)2870-2876
Number of pages7
JournalSmall
Volume6
Issue number24
DOIs
Publication statusPublished - 2010

Keywords

  • METIS-269956
  • IR-92557

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