VyCAP's puncher technology for single cell identification, isolation, and analysis

Michiel Stevens, Lisa Oomens, Joska Johannes Broekmaat, Joris Weersink, Fekri Abali, Joost Franciscus Swennenhuis, Arjan G.J. Tibbe (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Here we present the Puncher technology for the isolation of single cells. This technology combines a silicon chip with microwells, fluorescence imaging, and a punching method to isolate and transfer the single cells to standard reaction tubes. The technology is compatible with commercially available downstream workflows and instrumentation. Here we focus on the isolation of CTC but the Puncher technology can be applied to isolate single cells from liquid biopsies and more general from cell suspensions. It is especially suited for cell suspensions that contain:
* Cells of interest at a frequency of 1 per 10,000 or less
* A low total number of cells ranging from 1 to 100,000, that are present in a volume of 0.01 to 50 mL.

The frequency of appearance of CTC in blood is in the order of the 1 per 106 leukocytes. To be able to isolate the single CTC with the Puncher technology, enrichment of the CTC by a 3 logs reduction of the leukocytes is required. Here we describe the use of Rosettesep and Parsortix as examples of pre‐enrichment methods that are compatible with the Puncher technology and further downstream applications. © 2018 International Society for Advancement of Cytometry
Over the last couple of decades, multiple technologies have been developed for the enrichment and isolation of rare cells, including circulating tumor cell (CTC). Compatibility between these technologies, is however mostly not present 1-3. Enriched sample volumes are too large or the number of unwanted cells in the enriched samples is too high to identify and isolate the cells of interest. This leads to low recovery rates when isolating single CTC from the enriched samples. The Puncher technology for single cell isolation overcomes most of these issues.
Original languageEnglish
Pages (from-to)1255-1259
Number of pages5
JournalCytometry. Part A
Volume93A
Issue number12
DOIs
Publication statusPublished - 14 Nov 2018

Fingerprint

Cell Separation
Circulating Neoplastic Cells
Technology
Suspensions
Leukocytes
Cell Count
Workflow
Optical Imaging
Silicon
Biopsy

Keywords

  • Puncher
  • circulating tumor cells
  • clonal expansion
  • microwells
  • single cell isolation

Cite this

@article{58cef521c7c441669c7b76b6c3d20a4a,
title = "VyCAP's puncher technology for single cell identification, isolation, and analysis",
abstract = "Here we present the Puncher technology for the isolation of single cells. This technology combines a silicon chip with microwells, fluorescence imaging, and a punching method to isolate and transfer the single cells to standard reaction tubes. The technology is compatible with commercially available downstream workflows and instrumentation. Here we focus on the isolation of CTC but the Puncher technology can be applied to isolate single cells from liquid biopsies and more general from cell suspensions. It is especially suited for cell suspensions that contain: * Cells of interest at a frequency of 1 per 10,000 or less* A low total number of cells ranging from 1 to 100,000, that are present in a volume of 0.01 to 50 mL.The frequency of appearance of CTC in blood is in the order of the 1 per 106 leukocytes. To be able to isolate the single CTC with the Puncher technology, enrichment of the CTC by a 3 logs reduction of the leukocytes is required. Here we describe the use of Rosettesep and Parsortix as examples of pre‐enrichment methods that are compatible with the Puncher technology and further downstream applications. {\circledC} 2018 International Society for Advancement of CytometryOver the last couple of decades, multiple technologies have been developed for the enrichment and isolation of rare cells, including circulating tumor cell (CTC). Compatibility between these technologies, is however mostly not present 1-3. Enriched sample volumes are too large or the number of unwanted cells in the enriched samples is too high to identify and isolate the cells of interest. This leads to low recovery rates when isolating single CTC from the enriched samples. The Puncher technology for single cell isolation overcomes most of these issues.",
keywords = "Puncher, circulating tumor cells, clonal expansion, microwells, single cell isolation",
author = "Michiel Stevens and Lisa Oomens and Broekmaat, {Joska Johannes} and Joris Weersink and Fekri Abali and Swennenhuis, {Joost Franciscus} and Tibbe, {Arjan G.J.}",
year = "2018",
month = "11",
day = "14",
doi = "10.1002/cyto.a.23631",
language = "English",
volume = "93A",
pages = "1255--1259",
journal = "Cytometry. Part A",
issn = "1552-4922",
publisher = "Wiley-Liss Inc.",
number = "12",

}

VyCAP's puncher technology for single cell identification, isolation, and analysis. / Stevens, Michiel ; Oomens, Lisa ; Broekmaat, Joska Johannes; Weersink, Joris ; Abali, Fekri ; Swennenhuis, Joost Franciscus; Tibbe, Arjan G.J. (Corresponding Author).

In: Cytometry. Part A, Vol. 93A, No. 12, 14.11.2018, p. 1255-1259.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - VyCAP's puncher technology for single cell identification, isolation, and analysis

AU - Stevens, Michiel

AU - Oomens, Lisa

AU - Broekmaat, Joska Johannes

AU - Weersink, Joris

AU - Abali, Fekri

AU - Swennenhuis, Joost Franciscus

AU - Tibbe, Arjan G.J.

PY - 2018/11/14

Y1 - 2018/11/14

N2 - Here we present the Puncher technology for the isolation of single cells. This technology combines a silicon chip with microwells, fluorescence imaging, and a punching method to isolate and transfer the single cells to standard reaction tubes. The technology is compatible with commercially available downstream workflows and instrumentation. Here we focus on the isolation of CTC but the Puncher technology can be applied to isolate single cells from liquid biopsies and more general from cell suspensions. It is especially suited for cell suspensions that contain: * Cells of interest at a frequency of 1 per 10,000 or less* A low total number of cells ranging from 1 to 100,000, that are present in a volume of 0.01 to 50 mL.The frequency of appearance of CTC in blood is in the order of the 1 per 106 leukocytes. To be able to isolate the single CTC with the Puncher technology, enrichment of the CTC by a 3 logs reduction of the leukocytes is required. Here we describe the use of Rosettesep and Parsortix as examples of pre‐enrichment methods that are compatible with the Puncher technology and further downstream applications. © 2018 International Society for Advancement of CytometryOver the last couple of decades, multiple technologies have been developed for the enrichment and isolation of rare cells, including circulating tumor cell (CTC). Compatibility between these technologies, is however mostly not present 1-3. Enriched sample volumes are too large or the number of unwanted cells in the enriched samples is too high to identify and isolate the cells of interest. This leads to low recovery rates when isolating single CTC from the enriched samples. The Puncher technology for single cell isolation overcomes most of these issues.

AB - Here we present the Puncher technology for the isolation of single cells. This technology combines a silicon chip with microwells, fluorescence imaging, and a punching method to isolate and transfer the single cells to standard reaction tubes. The technology is compatible with commercially available downstream workflows and instrumentation. Here we focus on the isolation of CTC but the Puncher technology can be applied to isolate single cells from liquid biopsies and more general from cell suspensions. It is especially suited for cell suspensions that contain: * Cells of interest at a frequency of 1 per 10,000 or less* A low total number of cells ranging from 1 to 100,000, that are present in a volume of 0.01 to 50 mL.The frequency of appearance of CTC in blood is in the order of the 1 per 106 leukocytes. To be able to isolate the single CTC with the Puncher technology, enrichment of the CTC by a 3 logs reduction of the leukocytes is required. Here we describe the use of Rosettesep and Parsortix as examples of pre‐enrichment methods that are compatible with the Puncher technology and further downstream applications. © 2018 International Society for Advancement of CytometryOver the last couple of decades, multiple technologies have been developed for the enrichment and isolation of rare cells, including circulating tumor cell (CTC). Compatibility between these technologies, is however mostly not present 1-3. Enriched sample volumes are too large or the number of unwanted cells in the enriched samples is too high to identify and isolate the cells of interest. This leads to low recovery rates when isolating single CTC from the enriched samples. The Puncher technology for single cell isolation overcomes most of these issues.

KW - Puncher

KW - circulating tumor cells

KW - clonal expansion

KW - microwells

KW - single cell isolation

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85056451190&origin=resultslist&sort=plf-f&src=s&st1=VyCAP%27s+puncher+technology+for+single+cell+identification%2c+isolation%2c+and+analysis&st2=&sid=578d93fad8d4312fe00c0cf512cdfa3f&sot=b&sdt=b&sl=97&s=TITLE-ABS-KEY%28VyCAP%27s+puncher+technology+for+single+cell+identification%2c+isolation%2c+and+analysis%29&relpos=0&citeCnt=0&searchTerm=

UR - http://www.scopus.com/inward/record.url?scp=85056451190&partnerID=8YFLogxK

U2 - 10.1002/cyto.a.23631

DO - 10.1002/cyto.a.23631

M3 - Article

VL - 93A

SP - 1255

EP - 1259

JO - Cytometry. Part A

JF - Cytometry. Part A

SN - 1552-4922

IS - 12

ER -