Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry

Takayuki Obara; Stefan Schlautmann; Han J.G.E. Gardeniers

Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry

Takayuki Obara^*, Stefan Schlautmann, Han J.G.E. Gardeniers

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Droplet-based microfluidic systems coupled to electrospray ionization (ESI) mass spectrometry (MS) has a number of advantages for the study of enzyme kinetics: minimal reagent consumption, a well-defined reaction time control, and label-free and multiplex detection analysis. However, most studies have been dependent on ESI sources integrated on chips, which can be a challenge for practical application. Here, we report a simple trick for coupling of a standard ESI source to a droplet-based microfluidic chip that can also be made with standard techniques. With the new coupling, we demonstrated a label-free kinetic analysis of an enzymatic reaction in 50 nL droplets.

Original language	English
Title of host publication	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publisher	The Chemical and Biological Microsystems Society
Pages	1762-1764
Number of pages	3
ISBN (Print)	9780979806452
Publication status	Published - 2012
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012 - Okinawa, Japan Duration: 28 Oct 2012 → 1 Nov 2012 Conference number: 16

Publication series

Name	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012
Abbreviated title	MicroTAS
Country/Territory	Japan
City	Okinawa
Period	28/10/12 → 1/11/12

Keywords

Coupler
Droplets
ESI-MS
Reaction kinetics

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Obara, T., Schlautmann, S., & Gardeniers, H. J. G. E. (2012). Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1762-1764). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). The Chemical and Biological Microsystems Society.

Obara, Takayuki ; Schlautmann, Stefan ; Gardeniers, Han J.G.E. / Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. The Chemical and Biological Microsystems Society, 2012. pp. 1762-1764 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

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title = "Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry",

abstract = "Droplet-based microfluidic systems coupled to electrospray ionization (ESI) mass spectrometry (MS) has a number of advantages for the study of enzyme kinetics: minimal reagent consumption, a well-defined reaction time control, and label-free and multiplex detection analysis. However, most studies have been dependent on ESI sources integrated on chips, which can be a challenge for practical application. Here, we report a simple trick for coupling of a standard ESI source to a droplet-based microfluidic chip that can also be made with standard techniques. With the new coupling, we demonstrated a label-free kinetic analysis of an enzymatic reaction in 50 nL droplets.",

keywords = "Coupler, Droplets, ESI-MS, Reaction kinetics",

author = "Takayuki Obara and Stefan Schlautmann and Gardeniers, {Han J.G.E.}",

year = "2012",

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series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",

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booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",

note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012, MicroTAS ; Conference date: 28-10-2012 Through 01-11-2012",

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Obara, T, Schlautmann, S & Gardeniers, HJGE 2012, Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, The Chemical and Biological Microsystems Society, pp. 1762-1764, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012, Okinawa, Japan, 28/10/12.

Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry. / Obara, Takayuki; Schlautmann, Stefan ; Gardeniers, Han J.G.E.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. The Chemical and Biological Microsystems Society, 2012. p. 1762-1764 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry

AU - Obara, Takayuki

AU - Schlautmann, Stefan

AU - Gardeniers, Han J.G.E.

N1 - Conference code: 16

PY - 2012

Y1 - 2012

N2 - Droplet-based microfluidic systems coupled to electrospray ionization (ESI) mass spectrometry (MS) has a number of advantages for the study of enzyme kinetics: minimal reagent consumption, a well-defined reaction time control, and label-free and multiplex detection analysis. However, most studies have been dependent on ESI sources integrated on chips, which can be a challenge for practical application. Here, we report a simple trick for coupling of a standard ESI source to a droplet-based microfluidic chip that can also be made with standard techniques. With the new coupling, we demonstrated a label-free kinetic analysis of an enzymatic reaction in 50 nL droplets.

AB - Droplet-based microfluidic systems coupled to electrospray ionization (ESI) mass spectrometry (MS) has a number of advantages for the study of enzyme kinetics: minimal reagent consumption, a well-defined reaction time control, and label-free and multiplex detection analysis. However, most studies have been dependent on ESI sources integrated on chips, which can be a challenge for practical application. Here, we report a simple trick for coupling of a standard ESI source to a droplet-based microfluidic chip that can also be made with standard techniques. With the new coupling, we demonstrated a label-free kinetic analysis of an enzymatic reaction in 50 nL droplets.

KW - Coupler

KW - Droplets

KW - ESI-MS

KW - Reaction kinetics

UR - http://www.scopus.com/inward/record.url?scp=84901750484&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84901750484

SN - 9780979806452

T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

SP - 1762

EP - 1764

BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

PB - The Chemical and Biological Microsystems Society

T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012

Y2 - 28 October 2012 through 1 November 2012

ER -

Obara T, Schlautmann S , Gardeniers HJGE. Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. The Chemical and Biological Microsystems Society. 2012. p. 1762-1764. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry

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