Water-in-oil droplet-based microfluidic system for enzymatic studies, coupled to off-chip electrospray ionization mass spectrometry

Takayuki Obara*, Stefan Schlautmann, Han J.G.E. Gardeniers

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Abstract

Droplet-based microfluidic systems coupled to electrospray ionization (ESI) mass spectrometry (MS) has a number of advantages for the study of enzyme kinetics: minimal reagent consumption, a well-defined reaction time control, and label-free and multiplex detection analysis. However, most studies have been dependent on ESI sources integrated on chips, which can be a challenge for practical application. Here, we report a simple trick for coupling of a standard ESI source to a droplet-based microfluidic chip that can also be made with standard techniques. With the new coupling, we demonstrated a label-free kinetic analysis of an enzymatic reaction in 50 nL droplets.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherThe Chemical and Biological Microsystems Society
Pages1762-1764
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012
Conference number: 16

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2012
Abbreviated titleMicroTAS
Country/TerritoryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Coupler
  • Droplets
  • ESI-MS
  • Reaction kinetics

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