Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent

Zhiyuan Zhong; C. Lin; Y. Ma; Mark A. Hempenius; M.C. Lok; M.M. Fretz; Johannes F.J. Engbersen; Gyula J. Vancso; W.E. Hennink; Jan Feijen

doi:10.1016/j.jconrel.2006.09.061

Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent

Zhiyuan Zhong, C. Lin, Y. Ma, Mark A. Hempenius, M.C. Lok, M.M. Fretz, Johannes F.J. Engbersen, Gyula J. Vancso, W.E. Hennink, Jan Feijen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

8 Citations (Scopus)

12 Downloads (Pure)

Abstract

The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery

Original language	Undefined
Title of host publication	Proceedings of the ninth European symposium on controlled drug delivery
Editors	W.E. Hennink, J. Feijen, T Sam
Publisher	Elsevier
Pages	81-83
DOIs	https://doi.org/10.1016/j.jconrel.2006.09.061
Publication status	Published - 5 Apr 2006
Event	9th European Symposium on Controlled Drug Delivery, ESCDD 2006 - Noordwijk aan Zee, Netherlands Duration: 5 Apr 2006 → 7 Apr 2006 Conference number: 9

Publication series

Name
Publisher	Elsevier
Number	2
Volume	116
ISSN (Print)	0168-3659
ISSN (Electronic)	1873-4995

Conference

Conference	9th European Symposium on Controlled Drug Delivery, ESCDD 2006
Abbreviated title	ESCDD
Country/Territory	Netherlands
City	Noordwijk aan Zee
Period	5/04/06 → 7/04/06

Keywords

METIS-236898
IR-71118

Access to Document

10.1016/j.jconrel.2006.09.061

Cite this

Zhong, Z., Lin, C., Ma, Y., Hempenius, M. A., Lok, M. C., Fretz, M. M., Engbersen, J. F. J., Vancso, G. J., Hennink, W. E., & Feijen, J. (2006). Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. In W. E. Hennink, J. Feijen, & T. Sam (Eds.), Proceedings of the ninth European symposium on controlled drug delivery (pp. 81-83). Elsevier. https://doi.org/10.1016/j.jconrel.2006.09.061

@inproceedings{e5757096e0784464ba547f494f03dd79,

title = "Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent",

abstract = "The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery",

keywords = "METIS-236898, IR-71118",

author = "Zhiyuan Zhong and C. Lin and Y. Ma and Hempenius, {Mark A.} and M.C. Lok and M.M. Fretz and Engbersen, {Johannes F.J.} and Vancso, {Gyula J.} and W.E. Hennink and Jan Feijen",

note = "Proceedings of the Ninth European Symposium on Controlled Drug Delivery ; 9th European Symposium on Controlled Drug Delivery, ESCDD 2006 ; Conference date: 05-04-2006 Through 07-04-2006",

year = "2006",

month = apr,

day = "5",

doi = "10.1016/j.jconrel.2006.09.061",

language = "Undefined",

publisher = "Elsevier",

number = "2",

pages = "81--83",

editor = "W.E. Hennink and J. Feijen and T Sam",

booktitle = "Proceedings of the ninth European symposium on controlled drug delivery",

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Zhong, Z, Lin, C, Ma, Y, Hempenius, MA, Lok, MC, Fretz, MM, Engbersen, JFJ, Vancso, GJ, Hennink, WE & Feijen, J 2006, Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. in WE Hennink, J Feijen & T Sam (eds), Proceedings of the ninth European symposium on controlled drug delivery. Elsevier, pp. 81-83, 9th European Symposium on Controlled Drug Delivery, ESCDD 2006, Noordwijk aan Zee, Netherlands, 5/04/06. https://doi.org/10.1016/j.jconrel.2006.09.061

Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. / Zhong, Zhiyuan; Lin, C.; Ma, Y. et al.
Proceedings of the ninth European symposium on controlled drug delivery. ed. / W.E. Hennink; J. Feijen; T Sam. Elsevier, 2006. p. 81-83.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent

AU - Zhong, Zhiyuan

AU - Lin, C.

AU - Ma, Y.

AU - Hempenius, Mark A.

AU - Lok, M.C.

AU - Fretz, M.M.

AU - Engbersen, Johannes F.J.

AU - Vancso, Gyula J.

AU - Hennink, W.E.

AU - Feijen, Jan

N1 - Conference code: 9

PY - 2006/4/5

Y1 - 2006/4/5

N2 - The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery

AB - The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery

KW - METIS-236898

KW - IR-71118

U2 - 10.1016/j.jconrel.2006.09.061

DO - 10.1016/j.jconrel.2006.09.061

M3 - Conference contribution

SP - 81

EP - 83

BT - Proceedings of the ninth European symposium on controlled drug delivery

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A2 - Feijen, J.

A2 - Sam, T

PB - Elsevier

T2 - 9th European Symposium on Controlled Drug Delivery, ESCDD 2006

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ER -