Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent

Zhiyuan Zhong, C. Lin, Y. Ma, Mark A. Hempenius, M.C. Lok, M.M. Fretz, Johannes F.J. Engbersen, Gyula J. Vancso, W.E. Hennink, Jan Feijen

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

8 Citations (Scopus)

Abstract

The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery
Original languageUndefined
Title of host publicationProceedings of the ninth European symposium on controlled drug delivery
EditorsW.E. Hennink, J. Feijen, T Sam
PublisherElsevier
Pages81-83
DOIs
Publication statusPublished - 5 Apr 2006
Event9th European Symposium on Controlled Drug Delivery, ESCDD 2006 - Noordwijk aan Zee, Netherlands
Duration: 5 Apr 20067 Apr 2006
Conference number: 9

Publication series

Name
PublisherElsevier
Number2
Volume116
ISSN (Print)0168-3659
ISSN (Electronic)1873-4995

Conference

Conference9th European Symposium on Controlled Drug Delivery, ESCDD 2006
Abbreviated titleESCDD
CountryNetherlands
CityNoordwijk aan Zee
Period5/04/067/04/06

Keywords

  • METIS-236898
  • IR-71118

Cite this

Zhong, Z., Lin, C., Ma, Y., Hempenius, M. A., Lok, M. C., Fretz, M. M., ... Feijen, J. (2006). Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. In W. E. Hennink, J. Feijen, & T. Sam (Eds.), Proceedings of the ninth European symposium on controlled drug delivery (pp. 81-83). Elsevier. https://doi.org/10.1016/j.jconrel.2006.09.061
Zhong, Zhiyuan ; Lin, C. ; Ma, Y. ; Hempenius, Mark A. ; Lok, M.C. ; Fretz, M.M. ; Engbersen, Johannes F.J. ; Vancso, Gyula J. ; Hennink, W.E. ; Feijen, Jan. / Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. Proceedings of the ninth European symposium on controlled drug delivery. editor / W.E. Hennink ; J. Feijen ; T Sam. Elsevier, 2006. pp. 81-83
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title = "Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent",
abstract = "The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery",
keywords = "METIS-236898, IR-71118",
author = "Zhiyuan Zhong and C. Lin and Y. Ma and Hempenius, {Mark A.} and M.C. Lok and M.M. Fretz and Engbersen, {Johannes F.J.} and Vancso, {Gyula J.} and W.E. Hennink and Jan Feijen",
note = "Proceedings of the Ninth European Symposium on Controlled Drug Delivery",
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language = "Undefined",
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editor = "W.E. Hennink and J. Feijen and T Sam",
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Zhong, Z, Lin, C, Ma, Y, Hempenius, MA, Lok, MC, Fretz, MM, Engbersen, JFJ, Vancso, GJ, Hennink, WE & Feijen, J 2006, Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. in WE Hennink, J Feijen & T Sam (eds), Proceedings of the ninth European symposium on controlled drug delivery. Elsevier, pp. 81-83, 9th European Symposium on Controlled Drug Delivery, ESCDD 2006, Noordwijk aan Zee, Netherlands, 5/04/06. https://doi.org/10.1016/j.jconrel.2006.09.061

Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. / Zhong, Zhiyuan; Lin, C.; Ma, Y.; Hempenius, Mark A.; Lok, M.C.; Fretz, M.M.; Engbersen, Johannes F.J.; Vancso, Gyula J.; Hennink, W.E.; Feijen, Jan.

Proceedings of the ninth European symposium on controlled drug delivery. ed. / W.E. Hennink; J. Feijen; T Sam. Elsevier, 2006. p. 81-83.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent

AU - Zhong, Zhiyuan

AU - Lin, C.

AU - Ma, Y.

AU - Hempenius, Mark A.

AU - Lok, M.C.

AU - Fretz, M.M.

AU - Engbersen, Johannes F.J.

AU - Vancso, Gyula J.

AU - Hennink, W.E.

AU - Feijen, Jan

N1 - Proceedings of the Ninth European Symposium on Controlled Drug Delivery

PY - 2006/4/5

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N2 - The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery

AB - The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery

KW - METIS-236898

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BT - Proceedings of the ninth European symposium on controlled drug delivery

A2 - Hennink, W.E.

A2 - Feijen, J.

A2 - Sam, T

PB - Elsevier

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Zhong Z, Lin C, Ma Y, Hempenius MA, Lok MC, Fretz MM et al. Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. In Hennink WE, Feijen J, Sam T, editors, Proceedings of the ninth European symposium on controlled drug delivery. Elsevier. 2006. p. 81-83 https://doi.org/10.1016/j.jconrel.2006.09.061