Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent

Zhiyuan Zhong; C. Lin; Y. Ma; Mark A. Hempenius; M.C. Lok; M.M. Fretz; Johannes F.J. Engbersen; Gyula J. Vancso; W.E. Hennink; Jan Feijen

doi:10.1016/j.jconrel.2006.09.061

Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent

Zhiyuan Zhong
, C. Lin
, Y. Ma
, Mark A. Hempenius
, M.C. Lok
, M.M. Fretz
, Johannes F.J. Engbersen
, Gyula J. Vancso
, W.E. Hennink
, Jan Feijen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

8 Citations (Scopus)

12 Downloads (Pure)

Abstract

The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery

Original language	Undefined
Title of host publication	Proceedings of the ninth European symposium on controlled drug delivery
Editors	W.E. Hennink, J. Feijen, T Sam
Publisher	Elsevier
Pages	81-83
DOIs	https://doi.org/10.1016/j.jconrel.2006.09.061
Publication status	Published - 5 Apr 2006
Event	9th European Symposium on Controlled Drug Delivery, ESCDD 2006 - Noordwijk aan Zee, Netherlands Duration: 5 Apr 2006 → 7 Apr 2006 Conference number: 9

Publication series

Name
Publisher	Elsevier
Number	2
Volume	116
ISSN (Print)	0168-3659
ISSN (Electronic)	1873-4995

Conference

Conference	9th European Symposium on Controlled Drug Delivery, ESCDD 2006
Abbreviated title	ESCDD
Country/Territory	Netherlands
City	Noordwijk aan Zee
Period	5/04/06 → 7/04/06

Keywords

METIS-236898
IR-71118

Access to Document

10.1016/j.jconrel.2006.09.061

Cite this

Zhong, Z., Lin, C., Ma, Y., Hempenius, M. A., Lok, M. C., Fretz, M. M., Engbersen, J. F. J., Vancso, G. J., Hennink, W. E., & Feijen, J. (2006). Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. In W. E. Hennink, J. Feijen, & T. Sam (Eds.), Proceedings of the ninth European symposium on controlled drug delivery (pp. 81-83). Elsevier. https://doi.org/10.1016/j.jconrel.2006.09.061

@inproceedings{e5757096e0784464ba547f494f03dd79,

title = "Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent",

abstract = "The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery",

keywords = "METIS-236898, IR-71118",

author = "Zhiyuan Zhong and C. Lin and Y. Ma and Hempenius, \{Mark A.\} and M.C. Lok and M.M. Fretz and Engbersen, \{Johannes F.J.\} and Vancso, \{Gyula J.\} and W.E. Hennink and Jan Feijen",

note = "Proceedings of the Ninth European Symposium on Controlled Drug Delivery ; 9th European Symposium on Controlled Drug Delivery, ESCDD 2006 ; Conference date: 05-04-2006 Through 07-04-2006",

year = "2006",

month = apr,

day = "5",

doi = "10.1016/j.jconrel.2006.09.061",

language = "Undefined",

publisher = "Elsevier",

number = "2",

pages = "81--83",

editor = "W.E. Hennink and J. Feijen and T Sam",

booktitle = "Proceedings of the ninth European symposium on controlled drug delivery",

address = "Spain",

}

Zhong, Z, Lin, C, Ma, Y, Hempenius, MA, Lok, MC, Fretz, MM, Engbersen, JFJ , Vancso, GJ, Hennink, WE & Feijen, J 2006, Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. in WE Hennink, J Feijen & T Sam (eds), Proceedings of the ninth European symposium on controlled drug delivery. Elsevier, pp. 81-83, 9th European Symposium on Controlled Drug Delivery, ESCDD 2006, Noordwijk aan Zee, Netherlands, 5/04/06. https://doi.org/10.1016/j.jconrel.2006.09.061

Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent. / Zhong, Zhiyuan; Lin, C.; Ma, Y. et al.
Proceedings of the ninth European symposium on controlled drug delivery. ed. / W.E. Hennink; J. Feijen; T Sam. Elsevier, 2006. p. 81-83.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Water-soluble cationic poly(ferrocenylsilane): an efficient DNA condensation and transfection agent

AU - Zhong, Zhiyuan

AU - Lin, C.

AU - Ma, Y.

AU - Hempenius, Mark A.

AU - Lok, M.C.

AU - Fretz, M.M.

AU - Engbersen, Johannes F.J.

AU - Vancso, Gyula J.

AU - Hennink, W.E.

AU - Feijen, Jan

N1 - Conference code: 9

PY - 2006/4/5

Y1 - 2006/4/5

N2 - The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery

AB - The DNA condensation and in vitro gene transfer properties of an organo-iron polymer, cationic poly(ferrocenylsilane), were investigated. Gel retardation assay, dynamic light scattering (DLS), atomic force microscopy (AFM) and zeta-potential measurements showed that cationic poly(ferrocenylsilane) is able to condense plasmid DNA into positively charged nano-sized particles at N/P ratios ≥ 2/1. These polyplexes displayed low cytotoxicity and were able to efficiently transfect COS-7 cells. Keywords: poly(ferrocenylsilane), plasmid DNA, polyplexes, gene delivery

KW - METIS-236898

KW - IR-71118

U2 - 10.1016/j.jconrel.2006.09.061

DO - 10.1016/j.jconrel.2006.09.061

M3 - Conference contribution

SP - 81

EP - 83

BT - Proceedings of the ninth European symposium on controlled drug delivery

A2 - Hennink, W.E.

A2 - Feijen, J.

A2 - Sam, T

PB - Elsevier

T2 - 9th European Symposium on Controlled Drug Delivery, ESCDD 2006

Y2 - 5 April 2006 through 7 April 2006

ER -