White Blood Cell Differentiation Using a Solid State Flow Cytometer

R.M.P. Doornbos, E.J. Hennink, C.A.J. Putman, B.G. de Grooth, J. Greve

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    Abstract

    A flow cytometer using a solid state light source and detector was designed and built. For illumination of the sample stream two types of diode lasers (670 nm and 780 nm) were tested in a set-up designed to differentiate human leukocytes by means of light scattering. The detector is an avalanche photodiode, which was used to detect the weak scattered light in the orthogonal direction. The new flow cytometer set-up is very small, relatively cheap and yields similar results as a standard flow cytometer set-up using a helium-neon laser and photomultipliers.
    Original languageEnglish
    Pages (from-to)589-594
    Number of pages6
    JournalCytometry
    Volume14
    Issue number6
    DOIs
    Publication statusPublished - 1993

    Keywords

    • Differential white blood cell count
    • Avalanche photodiode
    • Instrumentation
    • Diode laser
    • Light scattering
    • Flow cytometry
    • Immunofluorescence
    • Miniaturization

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  • Cite this

    Doornbos, R. M. P., Hennink, E. J., Putman, C. A. J., de Grooth, B. G., & Greve, J. (1993). White Blood Cell Differentiation Using a Solid State Flow Cytometer. Cytometry, 14(6), 589-594. https://doi.org/10.1002/cyto.990140602