White Blood Cell Differentiation Using a Solid State Flow Cytometer

R.M.P. Doornbos, E.J. Hennink, C.A.J. Putman, B.G. de Grooth, J. Greve

    Research output: Contribution to journalArticleAcademicpeer-review

    7 Citations (Scopus)
    101 Downloads (Pure)

    Abstract

    A flow cytometer using a solid state light source and detector was designed and built. For illumination of the sample stream two types of diode lasers (670 nm and 780 nm) were tested in a set-up designed to differentiate human leukocytes by means of light scattering. The detector is an avalanche photodiode, which was used to detect the weak scattered light in the orthogonal direction. The new flow cytometer set-up is very small, relatively cheap and yields similar results as a standard flow cytometer set-up using a helium-neon laser and photomultipliers.
    Original languageEnglish
    Pages (from-to)589-594
    Number of pages6
    JournalCytometry
    Volume14
    Issue number6
    DOIs
    Publication statusPublished - 1993

    Fingerprint

    Cell Differentiation
    Leukocytes
    Light
    Avalanches
    Neon
    Semiconductor Lasers
    Gas Lasers
    Lighting
    Direction compound

    Keywords

    • differential white blood cell count
    • avalanche photodiode
    • METIS-129393
    • Instrumentation
    • Diode laser
    • IR-60726
    • Light scattering
    • Flow cytometry
    • immunofluorescence
    • Miniaturization

    Cite this

    Doornbos, R. M. P., Hennink, E. J., Putman, C. A. J., de Grooth, B. G., & Greve, J. (1993). White Blood Cell Differentiation Using a Solid State Flow Cytometer. Cytometry, 14(6), 589-594. https://doi.org/10.1002/cyto.990140602
    Doornbos, R.M.P. ; Hennink, E.J. ; Putman, C.A.J. ; de Grooth, B.G. ; Greve, J. / White Blood Cell Differentiation Using a Solid State Flow Cytometer. In: Cytometry. 1993 ; Vol. 14, No. 6. pp. 589-594.
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    abstract = "A flow cytometer using a solid state light source and detector was designed and built. For illumination of the sample stream two types of diode lasers (670 nm and 780 nm) were tested in a set-up designed to differentiate human leukocytes by means of light scattering. The detector is an avalanche photodiode, which was used to detect the weak scattered light in the orthogonal direction. The new flow cytometer set-up is very small, relatively cheap and yields similar results as a standard flow cytometer set-up using a helium-neon laser and photomultipliers.",
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    Doornbos, RMP, Hennink, EJ, Putman, CAJ, de Grooth, BG & Greve, J 1993, 'White Blood Cell Differentiation Using a Solid State Flow Cytometer' Cytometry, vol. 14, no. 6, pp. 589-594. https://doi.org/10.1002/cyto.990140602

    White Blood Cell Differentiation Using a Solid State Flow Cytometer. / Doornbos, R.M.P.; Hennink, E.J.; Putman, C.A.J.; de Grooth, B.G.; Greve, J.

    In: Cytometry, Vol. 14, No. 6, 1993, p. 589-594.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Doornbos, R.M.P.

    AU - Hennink, E.J.

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    AU - Greve, J.

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    AB - A flow cytometer using a solid state light source and detector was designed and built. For illumination of the sample stream two types of diode lasers (670 nm and 780 nm) were tested in a set-up designed to differentiate human leukocytes by means of light scattering. The detector is an avalanche photodiode, which was used to detect the weak scattered light in the orthogonal direction. The new flow cytometer set-up is very small, relatively cheap and yields similar results as a standard flow cytometer set-up using a helium-neon laser and photomultipliers.

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    KW - avalanche photodiode

    KW - METIS-129393

    KW - Instrumentation

    KW - Diode laser

    KW - IR-60726

    KW - Light scattering

    KW - Flow cytometry

    KW - immunofluorescence

    KW - Miniaturization

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    Doornbos RMP, Hennink EJ, Putman CAJ, de Grooth BG, Greve J. White Blood Cell Differentiation Using a Solid State Flow Cytometer. Cytometry. 1993;14(6):589-594. https://doi.org/10.1002/cyto.990140602