ZnO nano-rod devices for intradermal delivery and immunization

Tapas R. Nayak, Hao wang, Aakansha Pant, Minrui Zheng, Hans Junginger, Wei Jiang Goh, Choon Keong Lee, Shui Zou, Sylvie Alonso, Bertrand M.S. Czarny, Gert Storm, Chorng Haur Sow, Chengkuo Lee, Giorgia Pastorin

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Abstract

Intradermal delivery of antigens for vaccination is a very attractive approach since the skin provides a rich network of antigen presenting cells, which aid in stimulating an immune response. Numerous intradermal techniques have been developed to enhance penetration across the skin. However, these methods are invasive and/or affect the skin integrity. Hence, our group has devised zinc oxide (ZnO) nano-rods for non-destructive drug delivery. Chemical vapour deposition was used to fabricate aligned nano-rods on ZnO pre-coated silicon chips. The nano-rods’ length and diameter were found to depend on the temperature, time, quality of sputtered silicon chips, etc. Vertically aligned ZnO nano-rods with lengths of 30–35 µm and diameters of 200–300 nm were selected for in vitro human skin permeation studies using Franz cells with Albumin-fluorescein isothiocyanate (FITC) absorbed on the nano-rods. Fluorescence and confocal studies on the skin samples showed FITC penetration through the skin along the channels formed by the nano-rods. Bradford protein assay on the collected fluid samples indicated a significant quantity of Albumin-FITC in the first 12 h. Low antibody titres were observed with immunisation on Balb/c mice with ovalbumin (OVA) antigen coated on the nano-rod chips. Nonetheless, due to the reduced dimensions of the nano-rods, our device offers the additional advantage of excluding the simultaneous entrance of microbial pathogens. Taken together, these results showed that ZnO nano-rods hold the potential for a safe, non-invasive, and painless intradermal drug delivery.

Original languageEnglish
Article number147
JournalNanomaterials
Volume7
Issue number6
DOIs
Publication statusPublished - 15 Jun 2017
Externally publishedYes

Fingerprint

Immunization
Zinc Oxide
Zinc oxide
Skin
Fluorescein
Silicon
Antigens
Drug delivery
Albumins
Ovalbumin
Pathogens
Antibodies
Permeation
Chemical vapor deposition
Assays
Fluorescence
Proteins
Fluids
isothiocyanic acid

Keywords

  • Intradermal delivery
  • Skin immunization
  • ZnO nano-rods

Cite this

Nayak, T. R., wang, H., Pant, A., Zheng, M., Junginger, H., Goh, W. J., ... Pastorin, G. (2017). ZnO nano-rod devices for intradermal delivery and immunization. Nanomaterials, 7(6), [147]. https://doi.org/10.3390/nano7060147
Nayak, Tapas R. ; wang, Hao ; Pant, Aakansha ; Zheng, Minrui ; Junginger, Hans ; Goh, Wei Jiang ; Lee, Choon Keong ; Zou, Shui ; Alonso, Sylvie ; Czarny, Bertrand M.S. ; Storm, Gert ; Sow, Chorng Haur ; Lee, Chengkuo ; Pastorin, Giorgia. / ZnO nano-rod devices for intradermal delivery and immunization. In: Nanomaterials. 2017 ; Vol. 7, No. 6.
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abstract = "Intradermal delivery of antigens for vaccination is a very attractive approach since the skin provides a rich network of antigen presenting cells, which aid in stimulating an immune response. Numerous intradermal techniques have been developed to enhance penetration across the skin. However, these methods are invasive and/or affect the skin integrity. Hence, our group has devised zinc oxide (ZnO) nano-rods for non-destructive drug delivery. Chemical vapour deposition was used to fabricate aligned nano-rods on ZnO pre-coated silicon chips. The nano-rods’ length and diameter were found to depend on the temperature, time, quality of sputtered silicon chips, etc. Vertically aligned ZnO nano-rods with lengths of 30–35 µm and diameters of 200–300 nm were selected for in vitro human skin permeation studies using Franz cells with Albumin-fluorescein isothiocyanate (FITC) absorbed on the nano-rods. Fluorescence and confocal studies on the skin samples showed FITC penetration through the skin along the channels formed by the nano-rods. Bradford protein assay on the collected fluid samples indicated a significant quantity of Albumin-FITC in the first 12 h. Low antibody titres were observed with immunisation on Balb/c mice with ovalbumin (OVA) antigen coated on the nano-rod chips. Nonetheless, due to the reduced dimensions of the nano-rods, our device offers the additional advantage of excluding the simultaneous entrance of microbial pathogens. Taken together, these results showed that ZnO nano-rods hold the potential for a safe, non-invasive, and painless intradermal drug delivery.",
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Nayak, TR, wang, H, Pant, A, Zheng, M, Junginger, H, Goh, WJ, Lee, CK, Zou, S, Alonso, S, Czarny, BMS, Storm, G, Sow, CH, Lee, C & Pastorin, G 2017, 'ZnO nano-rod devices for intradermal delivery and immunization' Nanomaterials, vol. 7, no. 6, 147. https://doi.org/10.3390/nano7060147

ZnO nano-rod devices for intradermal delivery and immunization. / Nayak, Tapas R.; wang, Hao; Pant, Aakansha; Zheng, Minrui; Junginger, Hans; Goh, Wei Jiang; Lee, Choon Keong; Zou, Shui; Alonso, Sylvie; Czarny, Bertrand M.S.; Storm, Gert; Sow, Chorng Haur; Lee, Chengkuo; Pastorin, Giorgia.

In: Nanomaterials, Vol. 7, No. 6, 147, 15.06.2017.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - ZnO nano-rod devices for intradermal delivery and immunization

AU - Nayak, Tapas R.

AU - wang, Hao

AU - Pant, Aakansha

AU - Zheng, Minrui

AU - Junginger, Hans

AU - Goh, Wei Jiang

AU - Lee, Choon Keong

AU - Zou, Shui

AU - Alonso, Sylvie

AU - Czarny, Bertrand M.S.

AU - Storm, Gert

AU - Sow, Chorng Haur

AU - Lee, Chengkuo

AU - Pastorin, Giorgia

PY - 2017/6/15

Y1 - 2017/6/15

N2 - Intradermal delivery of antigens for vaccination is a very attractive approach since the skin provides a rich network of antigen presenting cells, which aid in stimulating an immune response. Numerous intradermal techniques have been developed to enhance penetration across the skin. However, these methods are invasive and/or affect the skin integrity. Hence, our group has devised zinc oxide (ZnO) nano-rods for non-destructive drug delivery. Chemical vapour deposition was used to fabricate aligned nano-rods on ZnO pre-coated silicon chips. The nano-rods’ length and diameter were found to depend on the temperature, time, quality of sputtered silicon chips, etc. Vertically aligned ZnO nano-rods with lengths of 30–35 µm and diameters of 200–300 nm were selected for in vitro human skin permeation studies using Franz cells with Albumin-fluorescein isothiocyanate (FITC) absorbed on the nano-rods. Fluorescence and confocal studies on the skin samples showed FITC penetration through the skin along the channels formed by the nano-rods. Bradford protein assay on the collected fluid samples indicated a significant quantity of Albumin-FITC in the first 12 h. Low antibody titres were observed with immunisation on Balb/c mice with ovalbumin (OVA) antigen coated on the nano-rod chips. Nonetheless, due to the reduced dimensions of the nano-rods, our device offers the additional advantage of excluding the simultaneous entrance of microbial pathogens. Taken together, these results showed that ZnO nano-rods hold the potential for a safe, non-invasive, and painless intradermal drug delivery.

AB - Intradermal delivery of antigens for vaccination is a very attractive approach since the skin provides a rich network of antigen presenting cells, which aid in stimulating an immune response. Numerous intradermal techniques have been developed to enhance penetration across the skin. However, these methods are invasive and/or affect the skin integrity. Hence, our group has devised zinc oxide (ZnO) nano-rods for non-destructive drug delivery. Chemical vapour deposition was used to fabricate aligned nano-rods on ZnO pre-coated silicon chips. The nano-rods’ length and diameter were found to depend on the temperature, time, quality of sputtered silicon chips, etc. Vertically aligned ZnO nano-rods with lengths of 30–35 µm and diameters of 200–300 nm were selected for in vitro human skin permeation studies using Franz cells with Albumin-fluorescein isothiocyanate (FITC) absorbed on the nano-rods. Fluorescence and confocal studies on the skin samples showed FITC penetration through the skin along the channels formed by the nano-rods. Bradford protein assay on the collected fluid samples indicated a significant quantity of Albumin-FITC in the first 12 h. Low antibody titres were observed with immunisation on Balb/c mice with ovalbumin (OVA) antigen coated on the nano-rod chips. Nonetheless, due to the reduced dimensions of the nano-rods, our device offers the additional advantage of excluding the simultaneous entrance of microbial pathogens. Taken together, these results showed that ZnO nano-rods hold the potential for a safe, non-invasive, and painless intradermal drug delivery.

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Nayak TR, wang H, Pant A, Zheng M, Junginger H, Goh WJ et al. ZnO nano-rod devices for intradermal delivery and immunization. Nanomaterials. 2017 Jun 15;7(6). 147. https://doi.org/10.3390/nano7060147