Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering

Nina Yu Kostina, Sebastien Blanquer, Ognen Pop-Georgievski, Khosrow Rahimi, Barbara Dittrich, Anita Höcherl, Jiří Michálek, Dirk W. Grijpma, Cesar Rodriguez-Emmenegger (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Stereolithography-assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω-methacrylate poly(d,l-lactide-block-ethylene glycol-block- d,l-lactide) (MA-PDLLA-PEG-PDLLA-MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected porosity possessing extremely high specific area. Hydrogels are characterized by infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and laser scanning confocal microscopy (LSCM). The copolymerization with zwitterionic comonomer leads hydrogels with high equilibrium water content (EWC), up to 700% while maintaining mechanical robustness. The introduction of carboxybetaine yields excellent resistance to nonspecific protein adsorption while providing a facile way for specific biofunctionalization with a model protein, fluorescein isothiocyanate labeled bovine serum albumin (BSA). The homogeneous protein immobilization across the hydrogel pores prove the accessibility to the innermost pore volumes. The remarkably low protein adsorption combined with the interconnected nature of the porosity allowing fast diffusion of nutrient and waste product and the mimicry of bone trabecular, makes the hydrogels presented here highly attractive for tissue engineering.

Original languageEnglish
Article number1800403
JournalMacromolecular bioscience
Volume19
Issue number4
DOIs
Publication statusPublished - 1 Apr 2019

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Hydrogels
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Porosity
Proteins
Adsorption
Waste Products
Stereolithography
Photoelectron Spectroscopy
Printing
Ethylene Glycol
Methacrylates
Confocal microscopy
Hydrogel
Fourier Transform Infrared Spectroscopy
Bovine Serum Albumin
Fluorescein
Confocal Microscopy
Immobilization

Keywords

  • UT-Hybrid-D
  • hydrogels
  • stereolithography
  • zwitterionic
  • gyroid pore structure

Cite this

Kostina, N. Y., Blanquer, S., Pop-Georgievski, O., Rahimi, K., Dittrich, B., Höcherl, A., ... Rodriguez-Emmenegger, C. (2019). Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering. Macromolecular bioscience, 19(4), [1800403]. https://doi.org/10.1002/mabi.201800403
Kostina, Nina Yu ; Blanquer, Sebastien ; Pop-Georgievski, Ognen ; Rahimi, Khosrow ; Dittrich, Barbara ; Höcherl, Anita ; Michálek, Jiří ; Grijpma, Dirk W. ; Rodriguez-Emmenegger, Cesar. / Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering. In: Macromolecular bioscience. 2019 ; Vol. 19, No. 4.
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Kostina, NY, Blanquer, S, Pop-Georgievski, O, Rahimi, K, Dittrich, B, Höcherl, A, Michálek, J, Grijpma, DW & Rodriguez-Emmenegger, C 2019, 'Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering' Macromolecular bioscience, vol. 19, no. 4, 1800403. https://doi.org/10.1002/mabi.201800403

Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering. / Kostina, Nina Yu; Blanquer, Sebastien; Pop-Georgievski, Ognen; Rahimi, Khosrow; Dittrich, Barbara; Höcherl, Anita; Michálek, Jiří; Grijpma, Dirk W.; Rodriguez-Emmenegger, Cesar (Corresponding Author).

In: Macromolecular bioscience, Vol. 19, No. 4, 1800403, 01.04.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering

AU - Kostina, Nina Yu

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AU - Pop-Georgievski, Ognen

AU - Rahimi, Khosrow

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AU - Höcherl, Anita

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Kostina NY, Blanquer S, Pop-Georgievski O, Rahimi K, Dittrich B, Höcherl A et al. Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering. Macromolecular bioscience. 2019 Apr 1;19(4). 1800403. https://doi.org/10.1002/mabi.201800403