Abstract
Stereolithography-assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω-methacrylate poly(d,l-lactide-block-ethylene glycol-block- d,l-lactide) (MA-PDLLA-PEG-PDLLA-MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected porosity possessing extremely high specific area. Hydrogels are characterized by infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and laser scanning confocal microscopy (LSCM). The copolymerization with zwitterionic comonomer leads hydrogels with high equilibrium water content (EWC), up to 700% while maintaining mechanical robustness. The introduction of carboxybetaine yields excellent resistance to nonspecific protein adsorption while providing a facile way for specific biofunctionalization with a model protein, fluorescein isothiocyanate labeled bovine serum albumin (BSA). The homogeneous protein immobilization across the hydrogel pores prove the accessibility to the innermost pore volumes. The remarkably low protein adsorption combined with the interconnected nature of the porosity allowing fast diffusion of nutrient and waste product and the mimicry of bone trabecular, makes the hydrogels presented here highly attractive for tissue engineering.
| Original language | English |
|---|---|
| Article number | 1800403 |
| Journal | Macromolecular bioscience |
| Volume | 19 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Apr 2019 |
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Keywords
- UT-Hybrid-D
- hydrogels
- stereolithography
- zwitterionic
- gyroid pore structure
Cite this
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Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering. / Kostina, Nina Yu; Blanquer, Sebastien; Pop-Georgievski, Ognen; Rahimi, Khosrow; Dittrich, Barbara; Höcherl, Anita; Michálek, Jiří; Grijpma, Dirk W.; Rodriguez-Emmenegger, Cesar (Corresponding Author).
In: Macromolecular bioscience, Vol. 19, No. 4, 1800403, 01.04.2019.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering
AU - Kostina, Nina Yu
AU - Blanquer, Sebastien
AU - Pop-Georgievski, Ognen
AU - Rahimi, Khosrow
AU - Dittrich, Barbara
AU - Höcherl, Anita
AU - Michálek, Jiří
AU - Grijpma, Dirk W.
AU - Rodriguez-Emmenegger, Cesar
N1 - Wiley deal
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Stereolithography-assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω-methacrylate poly(d,l-lactide-block-ethylene glycol-block- d,l-lactide) (MA-PDLLA-PEG-PDLLA-MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected porosity possessing extremely high specific area. Hydrogels are characterized by infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and laser scanning confocal microscopy (LSCM). The copolymerization with zwitterionic comonomer leads hydrogels with high equilibrium water content (EWC), up to 700% while maintaining mechanical robustness. The introduction of carboxybetaine yields excellent resistance to nonspecific protein adsorption while providing a facile way for specific biofunctionalization with a model protein, fluorescein isothiocyanate labeled bovine serum albumin (BSA). The homogeneous protein immobilization across the hydrogel pores prove the accessibility to the innermost pore volumes. The remarkably low protein adsorption combined with the interconnected nature of the porosity allowing fast diffusion of nutrient and waste product and the mimicry of bone trabecular, makes the hydrogels presented here highly attractive for tissue engineering.
AB - Stereolithography-assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω-methacrylate poly(d,l-lactide-block-ethylene glycol-block- d,l-lactide) (MA-PDLLA-PEG-PDLLA-MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected porosity possessing extremely high specific area. Hydrogels are characterized by infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and laser scanning confocal microscopy (LSCM). The copolymerization with zwitterionic comonomer leads hydrogels with high equilibrium water content (EWC), up to 700% while maintaining mechanical robustness. The introduction of carboxybetaine yields excellent resistance to nonspecific protein adsorption while providing a facile way for specific biofunctionalization with a model protein, fluorescein isothiocyanate labeled bovine serum albumin (BSA). The homogeneous protein immobilization across the hydrogel pores prove the accessibility to the innermost pore volumes. The remarkably low protein adsorption combined with the interconnected nature of the porosity allowing fast diffusion of nutrient and waste product and the mimicry of bone trabecular, makes the hydrogels presented here highly attractive for tissue engineering.
KW - UT-Hybrid-D
KW - hydrogels
KW - stereolithography
KW - zwitterionic
KW - gyroid pore structure
UR - http://www.scopus.com/inward/record.url?scp=85060123101&partnerID=8YFLogxK
U2 - 10.1002/mabi.201800403
DO - 10.1002/mabi.201800403
M3 - Article
VL - 19
JO - Macromolecular bioscience
JF - Macromolecular bioscience
SN - 1616-5187
IS - 4
M1 - 1800403
ER -